Fever after death of gram-negative bacteria is caused by release of:

Physical methods of microbial control, such as autoclaving, dry heat, or filtration, can achieve sterilization by completely eliminating all forms of microbial life, including bacterial spores. In contrast, chemical methods, like alcohols, chlorine, or phenolic compounds, primarily disinfect surfaces by killing or inhibiting vegetative bacteria, fungi, and some viruses, but they do not reliably destroy highly resistant spores. The effectiveness of chemical disinfectants depends on concentration, contact time, and the presence of organic material. Therefore, while physical methods can sterilize under controlled conditions, chemical methods are generally limited to disinfection rather than true sterilization.

A. Oncogenic virus: Oncogenic viruses are viruses that can cause cancer by integrating their genetic material into the host cell’s genome. This integration can disrupt normal cellular regulatory genes, activate proto-oncogenes, or inactivate tumor suppressor genes, leading to uncontrolled cell proliferation. Examples include human papillomavirus (HPV), Epstein-Barr virus (EBV), and hepatitis B virus (HBV), all of which are associated with specific cancers.

B. Non-infectious virus: A non-infectious virus is one that cannot successfully enter host cells or replicate, often due to damage or incomplete structure. Such viruses do not integrate into host DNA and therefore do not contribute to cancer development.

C. Structural virus: Structural viruses refer to viruses categorized based on their morphology, such as icosahedral, helical, or enveloped forms. While structure influences infectivity and stability, it does not inherently determine whether a virus is oncogenic or capable of causing cancer.