In Griffith's experiments with Streptococcus pneumoniae, rough non-encapsulated streptococci were converted into smooth encapsulated streptococci in the presence of the heat-killed smooth encapsulated streptococci. Which microbial process had Griffith identified?
transduction
conjugation
transformation
cloning
The Correct Answer is C
A. Transduction: Transduction is gene transfer mediated by bacteriophages (viruses that infect bacteria); Griffith’s experiment did not involve phage activity.
B. Conjugation: Conjugation requires direct cell-to-cell contact and transfer of DNA (often plasmids) via a pilus; Griffith’s work did not depend on cell contact.
C. Transformation: Transformation is the uptake and incorporation of free DNA from the environment by a bacterium; Griffith observed non-encapsulated cells acquiring traits from heat-killed encapsulated cells, which is transformation.
D. Cloning: Cloning refers to producing genetically identical copies or isolating a gene in laboratory techniques; this term does not describe Griffith’s natural gene uptake observation.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
A. Eurytolerant: Refers to organisms that can survive and grow under a wide range of environmental conditions (e.g., temperature, pH, salinity).
B. Stenotolerant: Opposite of eurytolerant; can survive only within a narrow range of conditions.
C. Obligate: Indicates a strict requirement for a particular condition (e.g., obligate aerobe requires oxygen).
D. Facultative: Indicates flexibility with respect to certain conditions (e.g., facultative anaerobes can
Correct Answer is B
Explanation
A. Heterotroph: Heterotrophs obtain carbon from organic compounds; if this organism were a heterotroph it would require organic carbon in the medium (or be able to digest agar or other organics), which the description does not indicate.
B. Autotroph: Autotrophs fix carbon dioxide as their carbon source; increased growth under high CO₂ and growth on a mineral salts medium with no digestible organics strongly suggest a CO₂-fixing autotroph.
C. Chemotroph: Chemotrophs obtain energy from chemical compounds rather than light; this term describes energy source rather than carbon source and can overlap with autotrophy or heterotrophy, so the information given points more directly to carbon fixation (autotrophy).
D. Halophile: Halophiles require or tolerate very high salt concentrations for growth; although the medium contains salts, nothing indicates extreme salinity preference, and the CO₂ response points toward autotrophy rather than halophily.
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