Which of the following pieces of personal protective equipment should a phlebotomist wear to adhere to universal precautions in anticipation of body fluid splashes?

Choice A Reason:
Shaking the tubes vigorously can cause hemolysis, which is the rupture of red blood cells, leading to inaccurate test results. It is not recommended as it does not provide gentle mixing and can damage the blood cells.

Choice B Reason:
Moving the tubes in a figure-eight motion is not a standard practice for mixing blood samples. This method may not provide the consistent and gentle inversion needed to mix the anticoagulant with the blood effectively.

Choice C Reason:
Rolling the tubes between the palms of the hands 4 to 8 times after collection is the recommended method. This technique allows for gentle and thorough mixing of the blood with the anticoagulant, preventing clotting without causing hemolysis. The number of inversions ensures that the anticoagulant coats the inner surface of the tube and mixes with the blood, which is essential for accurate laboratory results.

Choice D Reason:
Passing the tubes from hand to hand is not an effective method for mixing blood samples. This action may not provide the necessary inversion and can lead to inadequate mixing, resulting in clotted specimens.

Choice A reason:
Expired tubes may lose their vacuum, which is necessary to draw the correct volume of blood into the tube. The vacuum in blood collection tubes ensures that the correct amount of blood is drawn. If the vacuum is lost, the blood will not be drawn into the tube properly, which can lead to underfilling and affect the test results.

Choice B reason:
Hemoconcentration refers to an increase in the concentration of cells and solids in the blood, usually because of a loss of plasma. While this can occur due to prolonged tourniquet application or dehydration, it is not directly caused by the use of expired tubes.

Choice C reason:
Hemolysis is the destruction of red blood cells, which can release hemoglobin and other intracellular components into the plasma. It can be caused by physical damage, such as from a needle during blood draw, but is not a consequence of using expired tubes. However, if an expired tube causes improper blood draw due to loss of vacuum, it could indirectly lead to hemolysis due to multiple attempts to draw blood.

Choice D reason:
An elevation of the red blood cell count would not be directly caused by using expired tubes. The red blood cell count is determined by the body's production and destruction of red blood cells, not by the collection method or equipment used.