What is a primer in DNA sequencing?
A short piece of double-stranded DNA that binds to the template DNA and acts as a "starter" for the polymerase.
A short piece of double-stranded DNA that binds to the primer and acts as a "starter" for the template.
A short piece of single-stranded DNA that binds to the template DNA and acts as a "starter" for the polymerase.
A short piece of single-stranded DNA that binds to the polymerase and acts as a "starter" for the template.
Correct Answer : C
A primer is a short single-stranded DNA fragment used in certain laboratory techniques, such as the polymerase chain reaction (PCR).
In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified.

Choice A) A short piece of double-stranded DNA that binds to the template DNA and acts as a “starter” for the polymerase is incorrect because primers are single-stranded, not double-stranded.
Choice B) A short piece of double-stranded DNA that binds to the primer and acts as a “starter” for the template is incorrect because it does not make sense for a primer to bind to itself.
Choice D) A short piece of single-stranded DNA that binds to the polymerase and acts as a “starter” for the template is incorrect because primers bind to the template DNA, not to the polymerase.
Note: DNA primers are used instead of RNA primers in DNA sequencing and PCR because DNA is more stable, specific, and compatible with the enzymes and processes involved in these techniques.
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Related Questions
Correct Answer is A
Explanation
The correct answer is choice A. Thymus.
The thymus is a primary lymphoid organ located in the mediastinum.
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It plays a key role in the maturation and differentiation of T-lymphocytes.
Choice B.
Parathyroid is incorrect because the parathyroid glands are small endocrine glands located in the neck that produce parathyroid hormone, which regulates calcium levels in the blood.
Choice C.
Adrenal is incorrect because the adrenal glands are endocrine glands located above the kidneys that produce hormones such as cortisol and adrenaline.
Choice D.
Pituitary is incorrect because the pituitary gland is an endocrine gland located at the base of the brain that produces hormones that regulate growth, metabolism, and reproductive functions.
Correct Answer is A
Explanation
The correct answer is choice A.
The spleen is an organ in the human body that is responsible for the removal of damaged red blood cells and the production of certain types of white blood cells.
Choice B is incorrect because the kidneys are responsible for filtering waste from the blood and regulating electrolyte balance.
Choice C is incorrect because the pancreas produces hormones and enzymes that aid in digestion.
Choice D is incorrect because the thyroid gland produces hormones that regulate metabolism.
Correct Answer is D
Explanation
The correct answer is choice D -Enhances glomerular filtration rate.
The medication prescribed to the patient is a diuretic, which removes water and electrolytes from the body by increasing urination 1.
This helps reduce fluid buildup in the body.
Choice A, Inhibits the renin-angiotensin-aldosterone system, is not the correct answer because it describes a different mechanism of action.
Choice B, Blocks beta receptors, is not the correct answer because it describes a different mechanism of action.
Choice C, Increases sodium and water reabsorption, is not the correct answer because it would have the opposite effect of reducing fluid buildup.
Correct Answer is C
Explanation
The correct answer is choice C. Substitution.
A substitution mutation is a type of point mutation where one base in the DNA sequence is replaced by another base.
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Choice A is incorrect because a deletion mutation occurs when one or more bases are removed from the DNA sequence.
Choice B is incorrect because an insertion mutation occurs when one or more bases are added to the DNA sequence.
Choice D is incorrect because an inversion mutation occurs when a segment of DNA is reversed within the chromosome.
Correct Answer is B
Explanation
The correct answer is choice B.
Maintaining normal body temperature and blood pressure.
Early identification and management of potential organ donors must take into consideration specific pathophysiologic changes for medical optimization 1.
The VIPPS (ventilation, infusion and pumping, pharmacological treatment, and specificities) strategy is a mnemonic method that brings together key aspects of the restoration of oxygen delivery to tissues during hemodynamic instability plus organ optimization strategies.
Choice A is incorrect because administering antibiotics to prevent infection is not the most important intervention to preserve organ viability.
Choice C is incorrect because providing emotional support to family members, while important, is not an intervention that directly affects organ viability.
Choice D is incorrect because applying eye drops and ointment to prevent corneal drying is not the most important intervention to preserve organ viability.
Correct Answer is A
Explanation
Viruses use the host’s replication processes to produce progeny virus particles.
Viruses are obligate intracellular parasites, meaning that they require a host cell to replicate.
They hijack the host cell’s machinery to produce new virus particles.
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Choice B is incorrect because viruses do not divide inside the host cell.
Instead, they use the host cell’s machinery to produce new virus particles.
Choice C is incorrect because viruses do not have their own replication processes and rely on the host cell for replication.
Choice D is incorrect because while some viruses may destroy the host cell during replication, this is not always the case and is not the primary relationship between viruses and their host cells during replication.
Correct Answer is A
Explanation
The neuromuscular junction is a type of synapse where neuronal signals from the brain or spinal cord interact with skeletal muscle fibers, causing them to contract.

The activation of many muscle fibers together causes muscles to contract, which in turn can produce movement.
Choice B is incorrect because binding acetylcholine to nAChRs is a process that occurs at the neuromuscular junction, but it is not the function of the neuromuscular junction itself.
Choice C is incorrect because depolarizing the muscle cell membrane is a result of the function of the neuromuscular junction, but it is not the function itself.
Choice D is incorrect because activating voltage-gated sodium channels on the muscle membrane is a result of the function of the neuromuscular junction, but it is not the function itself.
Correct Answer is A
Explanation
These are the four nucleotide bases found in DNA1.

Choice B) Adenine, Thymidine, Cytidine, Guanine is incorrect because Thymidine and Cytidine are not nucleotide bases found in DNA.
Choice C) Adenosine, Thymine, Cytosine, Guanosine is incorrect because Adenosine and Guanosine are not nucleotide bases found in DNA.
Choice D) Adenosine, Thymidine, Cytidine, Guanosine is incorrect because Adenosine, Thymidine and Cytidine are not nucleotide bases found in DNA.
Correct Answer is A
Explanation
The normal flora refers to the microbial community that colonizes on the skin and mucus membrane .
Normal flora can be found in many sites of the human body including the skin, respiratory tract, urinary tract, and the digestive tract.

Choice B is incorrect because normal flora does not refer to a group of infectious parasites that cause diarrheal diseases .
Choice C is incorrect because normal flora does not refer to the genetic material of bacteria housed within a true nucleus .
Choice D is incorrect because normal flora does not refer to the protein coat surrounding the viral genome .
Correct Answer is A
Explanation
The correct answer is choice A.
Increased respiration rate to remove excess CO2.
The body regulates blood pH through several mechanisms, including chemical buffers, the respiratory system, and the urinary system.
The respiratory system can adjust blood pH by changing the rate of respiration to remove or retain CO2.
When there is excess acid in the blood, the respiratory rate increases to remove more CO2, which helps to raise blood pH.
Choice B is incorrect because decreasing the respiration rate would retain CO2, which would lower blood pH.
Choice C is incorrect because increased water intake would not directly affect blood pH levels.
Choice D is incorrect because decreased water intake would not directly affect blood pH levels.
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