What is the purpose of using PCR (polymerase chain reaction) in the laboratory?

A control group is used in scientific studies to establish causality by isolating the effect of an independent variable.

The control group serves as a baseline or comparison group that does not receive the treatment or intervention being tested.

By comparing the results of the control group to the experimental group, researchers can determine if any observed changes are due to the independent variable or if they are due to chance or other factors.

Choice B is incorrect because a control group is not used to establish the effect of a dependent variable on an independent variable.

Choice C is incorrect because while a control group can help control for the impact of extraneous variables on the dependent variable, its primary purpose is to isolate the effect of the independent variable.

Choice D is incorrect because a control group is not used to control for the impact of extraneous variables on the independent variable.

The correct answer is choice C. Thyroxine.

Thyroxine (T4) is a hormone produced by the thyroid gland that controls your body’s metabolism, the process in which your body transforms the food you eat into energy.

Choice A, Estrogen, is not the correct answer because it is a hormone responsible for the development of female secondary sexual characteristics.

Choice B, Progestin, is not the correct answer because it is a synthetic form of progesterone used in hormonal birth control and hormone replacement therapy.

Choice D, Androgen, is not the correct answer because it is a hormone responsible for the development of male secondary sexual characteristics.

Concentration of solute particles in the solution.

Osmosis is the movement of water across a semipermeable membrane from an area of lower solute concentration to an area of higher solute concentration.

The concentration of solute particles in the solution is the primary factor that determines the movement of water across cell membranes in osmosis.

Hydrostatic pressure (choice A) can affect the movement of water across cell membranes but is not the primary factor responsible for osmosis.

Temperature (choice C) can affect the rate of osmosis but is not the primary factor responsible for osmosis.

Kinetic energy of liquid water molecules (choice D) can affect the rate of osmosis but is not the primary factor responsible for osmosis.

Protons contribute to the positive charge of an atom.

Protons are subatomic particles with a positive charge found in the nucleus of an atom.

Choice B is incorrect because neutrons are neutral and do not have a charge. Choice C is incorrect because electrons have a negative charge.

Choice D is incorrect because nucleons refer to both protons and neutrons, but only protons contribute to the positive charge of an atom.

A primer is a short single-stranded DNA fragment used in certain laboratory techniques, such as the polymerase chain reaction (PCR).

In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified.

Choice A) A short piece of double-stranded DNA that binds to the template DNA and acts as a “starter” for the polymerase is incorrect because primers are single-stranded, not double-stranded.

Choice B) A short piece of double-stranded DNA that binds to the primer and acts as a “starter” for the template is incorrect because it does not make sense for a primer to bind to itself.

Choice D) A short piece of single-stranded DNA that binds to the polymerase and acts as a “starter” for the template is incorrect because primers bind to the template DNA, not to the polymerase.

Note:DNA primers are used instead of RNA primers in DNA sequencing and PCR because DNA is more stable, specific, and compatible with the enzymes and processes involved in these techniques.

Proximal tubule

The proximal tubule is responsible for reabsorbing all the nutrients and most of the water.

Choice A is incorrect because the distal tubule is not primarily responsible for reabsorbing ions, water and nutrients.

Choice C is incorrect because the glomerulus is responsible for filtering fluid and solutes out of the blood to form a glomerular filtrate.

Choice D is incorrect because the Loop of Henle is not primarily responsible for reabsorbing ions, water and nutrients.

Granzymes.

Granzymes are a family of serine proteases that are stored in and secreted from the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells.

They work in synergy with perforin, a pore-forming toxin, to induce apoptosis in target cells.

Perforin is necessary for the delivery of granzyme B to the target cell cytosol where caspase-dependent and -independent pathways to apoptosis are activated.

Perforins (choice A) are pore-forming toxins that work in synergy with granzymes to induce apoptosis in target cells.

Cytokines (choice B) are signaling molecules that regulate immune responses but do not directly induce apoptosis in target cells.

Interferons (choice D) are a type of cytokine that play a role in immune responses but do not directly induce apoptosis in target cells.

Bacteria can perform photosynthesis while archaea cannot. Many types of bacteria can generate oxygen from sunlight through photosynthesis, while archaea cannot perform this process.

Choice A is incorrect because neither bacteria nor archaea have a true nucleus. Both are prokaryotic organisms. Choice B is incorrect because archaea reproduce by fission, fragmentation, or budding, while bacteria can produce spores and divide sexually or asexually. Choice D is incorrect because archaeal and bacterial flagella are constructed differently.

Corpus luteum.

During the menstrual cycle, the corpus luteum in the ovary produces progesterone to prepare the endometrium for potential implantation.

Choice B is incorrect because fimbriae are finger-like projections at the end of the fallopian tubes that help guide the egg into the tube.

Choice C is incorrect because a follicle is a sac in the ovary that contains an immature egg.

Choice D is incorrect because the ovarian ligament is a fibrous band of tissue that connects the ovary to the uterus.

The correct answer is choice A. Orthopnea.

Orthopnea refers to a condition in which a patient experiences difficulty breathing while lying down, but their breathing improves when they sit up or stand.

Choice B, Hypoxia, is not the correct answer because it refers to a condition in which there is a lack of oxygen supply to the body’s tissues.

Choice C, Tachypnea, is not the correct answer because it refers to rapid breathing.

Choice D, Bradypnea, is not the correct answer because it refers to abnormally slow breathing.