EMB agar is considered selective because:
It enhances growth of Gram-positive bacteria
It contains dyes that inhibit Gram-positive organisms
It only supports anaerobic growth
It contains high salt concentration
The Correct Answer is B
A. It enhances growth of Gram-positive bacteria: EMB agar does not promote the growth of Gram-positive bacteria; in fact, it inhibits them. Its formulation is designed to favor Gram-negative organisms, particularly enteric bacteria, by incorporating selective agents.
B. It contains dyes that inhibit Gram-positive organisms: EMB (Eosin Methylene Blue) agar contains the dyes eosin Y and methylene blue, which selectively inhibit the growth of most Gram-positive bacteria while allowing Gram-negative bacteria to grow. This selectivity makes EMB useful for isolating enteric Gram-negative bacilli and differentiating lactose fermenters from non-fermenters.
C. It only supports anaerobic growth: EMB agar supports the growth of facultative and aerobic Gram-negative bacteria. Anaerobic growth is not a specific feature of EMB, and oxygen is not restricted in its use.
D. It contains high salt concentration: High salt concentration is a characteristic of selective media like Mannitol Salt Agar (MSA), which inhibits non-halotolerant bacteria. EMB’s selectivity is based on dyes, not salt concentration, so this is not the mechanism for inhibiting Gram-positive bacteria.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
Coarse adjustment: The arrow points to the coarse adjustment knob, a large focusing knob on the microscope used to move the stage up or down quickly to bring the specimen into general focus. It is typically used with low-power objective lenses to locate the specimen and obtain an initial image. Using it with high-power lenses can risk damaging the slide or lens because it moves the stage in large increments.
Fine adjustment: A smaller focusing knob that moves the stage very slightly to sharpen the image after the specimen has been brought into general focus. It is mainly used with high-power or oil immersion objectives to achieve precise, clear visualization of cellular details without risking damage to the specimen.
Correct Answer is A
Explanation
Hemolysins are bacterial exotoxins that specifically target and lyse red blood cells, releasing hemoglobin into the surrounding environment. They are produced by certain pathogenic bacteria, such as Streptococcus and Staphylococcus species, and contribute to nutrient acquisition by providing iron from hemoglobin. Hemolysins can cause varying degrees of red blood cell destruction, resulting in different patterns on blood agar: alpha hemolysis (partial lysis with greenish discoloration), beta hemolysis (complete lysis with clear zones), and gamma hemolysis (no lysis). Their activity is an important virulence factor in bacterial infections.
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