To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
Titration
Electrophoresis
Filtration
Spectrophotometry
Correct Answer : B
Electrophoresis is the most useful laboratory method for separating genomic DNA fragments by size.
Electrophoresis is a technique that uses an electric field to separate charged molecules, such as DNA fragments, based on their size and charge.
Choice A is not correct because titration is a laboratory method used to determine the concentration of a solution.
Choice C is not correct because filtration is a laboratory method used to separate solids from liquids.
Choice D is not correct because spectrophotometry is a laboratory method used to measure the absorbance of light by a solution.
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Related Questions
Correct Answer is B
Explanation
The corpus luteum is a structure that develops in the ovary after an egg has been released.
It secretes the hormone progesterone, which prepares the uterus for a fertilized egg to implant and helps maintain the uterine lining during pregnancy1.

Choice A.
Umbilical cord is not correct because it is a structure that connects the developing fetus to the placenta and provides nutrients and oxygen to the fetus, but does not secrete hormones.
Choice C.
Oviduct is not correct because it is a tube that transports eggs from the ovary to the uterus, but does not secrete hormones.
Choice D.
Oocyte is not correct because it is an immature egg cell, but does not secrete hormones.
Correct Answer is D
Explanation
The polarity of water molecules explains its solvent abilities for certain substances.
Water is a polar molecule because it has a partial positive charge on one end and a partial negative charge on the other end due to the unequal sharing of electrons between the oxygen and hydrogen atoms.
This polarity allows water to dissolve other polar substances and ionic compounds.

Choice A.
Kinetic energy of liquid water molecules is not the correct answer because kinetic energy refers to the energy of motion and does not directly explain water’s solvent abilities.
Choice B.
High specific heat is not the correct answer because specific heat refers to the amount of heat required to raise the temperature of a substance and does not directly explain water’s solvent abilities.
Choice C.
High surface tension is not the correct answer because surface tension refers to the cohesive forces between liquid molecules and does not directly explain water’s solvent abilities.
Correct Answer is C
Explanation
Control.
A control group is a group in an experiment that does not receive the treatment or manipulation being tested and is used as a benchmark to measure how the other tested subjects do.
The control group is used to minimize the effects of all variables except the independent variable.
This allows researchers to determine if changes in the dependent variable are due to the manipulation of the independent variable or if they are due to some other factor.
Choice A.
Responding is not the correct answer because it refers to the dependent variable, which is the variable that is being measured in an experiment.
Choice B.
Manipulated is not the correct answer because it refers to the independent variable, which is the variable that is being manipulated in an experiment.
Choice D.
Variable is not the correct answer because it refers to any factor that can change in an experiment and can include both independent and dependent variables.
Correct Answer is B
Explanation
The hypothalamus is a small region of the brain located just above the brainstem. It serves as a critical control center for maintaining homeostasis — the body’s internal balance. One of its key roles is regulating core body temperature through a process called thermoregulation.
The hypothalamus monitors the body's temperature using temperature-sensitive neurons. If the body becomes too hot or too cold, the hypothalamus initiates responses to bring the temperature back to a normal range by:
- Triggering sweating to cool down the body.
- Initiating shivering to generate heat.
- Adjusting blood flow to the skin for heat loss or retention.
In addition to temperature regulation, the hypothalamus also controls other vital functions like:
- Hunger and thirst
- Sleep-wake cycles
- Hormone release (by influencing the pituitary gland)
- Stress response
Why the Other Options Are Incorrect:
- A. Pituitary gland: While the pituitary gland is known as the “master gland” because it controls other endocrine glands, it does so under the direction of the hypothalamus. It does not directly regulate body temperature.
- C. Adrenal gland: The adrenal glands produce hormones like cortisol and adrenaline, primarily involved in the stress response, not temperature regulation.
- D. Pancreas: The pancreas is mainly responsible for regulating blood sugar levels through insulin and glucagon, not body temperature.
Thus, the hypothalamus is the part of the endocrine system most responsible for maintaining homeostasis, including regulating core body temperature.
Correct Answer is B
Explanation
The hypothesis should be modified to include the new findings that worm infestation can relieve the effects of certain autoimmune disorders.
A possible modification could be: “Parasitic worm infestation can have both damaging and beneficial effects on the host.
While it can cause harm, it has also been found to reduce the severity of certain autoimmune disorders.”
Choice A.
Worm infestation prevents the body from immune malfunction is not correct because it overstates the findings and implies that worm infestation completely prevents immune malfunction, which is not supported by the evidence.
Choice C.
Worm infestations exacerbate the body’s immune reactions is not correct because it contradicts the new findings that worm infestation can relieve the effects of certain autoimmune disorders.
Choice D.
Lack of worm infestations is the cause of some autoimmune disorders is not correct because it overstates the findings and implies a causal relationship between lack of worm infestations and autoimmune disorders, which is not supported by the evidence.
Correct Answer is B
Explanation
Diffusion down a concentration gradient causes most of the carbon dioxide from the blood to move into the alveoli.
The alveoli are tiny air sacs in the lungs where gas exchange occurs.
Carbon dioxide is a waste product of cellular respiration and is carried by the blood to the lungs to be exhaled.
In the lungs, carbon dioxide diffuses from the blood (where its concentration is high) into the alveoli (where its concentration is lower) down its concentration gradient.

Choice A is incorrect because carbon dioxide is not converted to carbon monoxide in the body.
Choice C is incorrect because passive transport using carrier proteins is not the primary mechanism by which carbon dioxide moves from the blood into the alveoli.
Choice D is incorrect because active transport using energy is not involved in the movement of carbon dioxide from the blood into the alveoli.
Correct Answer is C
Explanation
Osmosis is the movement of water molecules across a selectively permeable membrane from an area of higher water concentration to an area of lower water concentration.
In a hypertonic solution, the concentration of solutes outside the cell is higher than inside the cell, so water flows out of the cell through aquaporins embedded in the plasma membrane to balance the concentration gradient.

Choice A.
Facilitated diffusion is not correct because it is a type of passive transport that involves the movement of molecules across a membrane through specific transport proteins, but it does not specifically refer to the movement of water molecules.
Choice B.
Active transport is not correct because it is a type of transport that involves the movement of molecules against their concentration gradient and requires energy in the form of ATP, but osmosis is a passive process that does not require energy.
Choice D.
Diffusion is not correct because it refers to the movement of molecules from an area of higher concentration to an area of lower concentration, but it does not specifically refer to the movement of water molecules.
Correct Answer is D
Explanation
Microtubule organization.
Centrosomes are organelles that serve as the main microtubule-organizing centers for animal cells.
They regulate the movement of microtubules and other cytoskeletal structures, thereby facilitating changes in the shapes of the membranes of animal cells.

Choice A, Organelle trafficking, is not the correct answer because while centrosomes do play a role in intracellular trafficking during interphase by organizing an astral ray of microtubules, their main function is microtubule organization.
Choice B, Pathogen digestion, is not the correct answer because centrosomes do not play a direct role in pathogen digestion.
Choice C, Cytoplasm formation, is not the correct answer because centrosomes do not play a direct role in cytoplasm formation.
Correct Answer is B
Explanation
The atomic number of an atom is equal to the number of protons in its nucleus.
In this case, the atom has 12 protons, so its atomic number is 12.

Choice A, 24, is not the correct answer because it represents the sum of the number of protons and neutrons in the atom’s nucleus, which is known as the mass number.
Choice C, 1, is not the correct answer because it does not represent the number of protons in the atom’s nucleus.
Choice D, 144, is not the correct answer because it represents the square of the mass number and does not represent any property of the atom.
Correct Answer is C
Explanation
Amino acids have a unique structure consisting of an amino group (-NH3⁺) and a carboxyl group (-COO⁻) attached to a central carbon (called the α-carbon). At physiological pH (around 7.4), these functional groups often exist in their ionized forms:
- The amino group (-NH3⁺) is positively charged, acting as a proton acceptor (a base).
- The carboxyl group (-COO⁻) is negatively charged, acting as a proton donor (an acid).
This results in a zwitterion — a molecule with both a positive and a negative charge. Because amino acids can accept or donate protons depending on the pH of their environment, they have buffering capacity. This means they can resist changes in pH by stabilizing the concentration of hydrogen ions (H⁺).
Why Other Options Are Incorrect:
- A. Monosaccharides: These are simple sugars without ionizable functional groups, so they cannot act as buffers.
- B. Ribonucleotides and D. Deoxyribonucleotides: While nucleotides have phosphate groups that can donate protons, they lack the dual positive and negative functional groups necessary for the strong buffering effect seen in amino acids.
Therefore, amino acids are the correct choice because their zwitterionic nature provides them with excellent buffering capacity.
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