To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
Titration
Electrophoresis
Filtration
Spectrophotometry
Correct Answer : B
Electrophoresis is the most useful laboratory method for separating genomic DNA fragments by size.
Electrophoresis is a technique that uses an electric field to separate charged molecules, such as DNA fragments, based on their size and charge.
Choice A is not correct because titration is a laboratory method used to determine the concentration of a solution.
Choice C is not correct because filtration is a laboratory method used to separate solids from liquids.
Choice D is not correct because spectrophotometry is a laboratory method used to measure the absorbance of light by a solution.
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Related Questions
Correct Answer is D
Explanation
Sodium bicarbonate neutralizes the acidity of chyme.
The pancreas secretes large amounts of sodium bicarbonate, which protects the duodenum by neutralizing the acid that comes from the stomach.
This compound helps neutralize stomach acid generated during the digestive process.

Choice A is incorrect because sodium bicarbonate is not a protease that digests carbohydrates.
Proteases are enzymes that break down proteins, while sodium bicarbonate is a chemical compound that helps neutralize stomach acid.
Choice B is incorrect because sodium bicarbonate does not stimulate the pyloric sphincter.
The pyloric sphincter is a ring of smooth muscle that separates the stomach from the duodenum and regulates the passage of partially digested food (chyme) into the small intestine.
Choice C is incorrect because sodium bicarbonate does not inhibit peristalsis.
Peristalsis is a series of wave-like muscle contractions that move food through the digestive tract.
Correct Answer is B
Explanation
The hypothalamus is a small region of the brain located just above the brainstem. It serves as a critical control center for maintaining homeostasis — the body’s internal balance. One of its key roles is regulating core body temperature through a process called thermoregulation.
The hypothalamus monitors the body's temperature using temperature-sensitive neurons. If the body becomes too hot or too cold, the hypothalamus initiates responses to bring the temperature back to a normal range by:
- Triggering sweating to cool down the body.
- Initiating shivering to generate heat.
- Adjusting blood flow to the skin for heat loss or retention.
In addition to temperature regulation, the hypothalamus also controls other vital functions like:
- Hunger and thirst
- Sleep-wake cycles
- Hormone release (by influencing the pituitary gland)
- Stress response
Why the Other Options Are Incorrect:
- A. Pituitary gland: While the pituitary gland is known as the “master gland” because it controls other endocrine glands, it does so under the direction of the hypothalamus. It does not directly regulate body temperature.
- C. Adrenal gland: The adrenal glands produce hormones like cortisol and adrenaline, primarily involved in the stress response, not temperature regulation.
- D. Pancreas: The pancreas is mainly responsible for regulating blood sugar levels through insulin and glucagon, not body temperature.
Thus, the hypothalamus is the part of the endocrine system most responsible for maintaining homeostasis, including regulating core body temperature.
Correct Answer is B
Explanation
The hypothesis should be modified to include the new findings that worm infestation can relieve the effects of certain autoimmune disorders.
A possible modification could be: “Parasitic worm infestation can have both damaging and beneficial effects on the host.
While it can cause harm, it has also been found to reduce the severity of certain autoimmune disorders.”
Choice A.
Worm infestation prevents the body from immune malfunction is not correct because it overstates the findings and implies that worm infestation completely prevents immune malfunction, which is not supported by the evidence.
Choice C.
Worm infestations exacerbate the body’s immune reactions is not correct because it contradicts the new findings that worm infestation can relieve the effects of certain autoimmune disorders.
Choice D.
Lack of worm infestations is the cause of some autoimmune disorders is not correct because it overstates the findings and implies a causal relationship between lack of worm infestations and autoimmune disorders, which is not supported by the evidence.
Correct Answer is D
Explanation
The polarity of water molecules explains its solvent abilities for certain substances.
Water is a polar molecule because it has a partial positive charge on one end and a partial negative charge on the other end due to the unequal sharing of electrons between the oxygen and hydrogen atoms.
This polarity allows water to dissolve other polar substances and ionic compounds.

Choice A.
Kinetic energy of liquid water molecules is not the correct answer because kinetic energy refers to the energy of motion and does not directly explain water’s solvent abilities.
Choice B.
High specific heat is not the correct answer because specific heat refers to the amount of heat required to raise the temperature of a substance and does not directly explain water’s solvent abilities.
Choice C.
High surface tension is not the correct answer because surface tension refers to the cohesive forces between liquid molecules and does not directly explain water’s solvent abilities.
Correct Answer is D
Explanation
Genes are used in the process of DNA sequencing to determine the order of nucleotides in a DNA molecule.

Choice B.
Blood types is not the correct answer because blood types are determined by the presence or absence of specific antigens on the surface of red blood cells and are not directly related to DNA sequencing.
Choice C.
Hormones is not the correct answer because hormones are chemical messengers produced by glands in the body and are not directly involved in DNA sequencing.
Choice D.
Genes is the correct answer because genes are used in the process of DNA sequencing to determine the order of nucleotides in a DNA molecule.
Correct Answer is A
Explanation
Urea is a substance that is excreted by sweat glands in response to the breakdown of proteins and the formation of ammonia.
When proteins are broken down, they produce ammonia, which is a highly toxic compound for the body.
Ammonia is then converted into urea and released out of the body through sweat glands.
Choice B.
Sebum is not correct because it is an oily substance secreted by sebaceous glands to lubricate and protect the skin, but it is not related to the breakdown of proteins or the formation of ammonia.
Choice C.
Water is not correct because while it is a component of sweat, it is not specifically related to the breakdown of proteins or the formation of ammonia.
Choice D.
Lysozymes are not correct because they are enzymes found in tears, saliva and other body fluids that have antibacterial properties, but they are not related to the breakdown of proteins or the formation of ammonia.
Correct Answer is A
Explanation
A myocardial infarction affects the coronary blood vessels of the heart.
A myocardial infarction, commonly known as a heart attack, occurs when blood flow decreases or stops in the coronary artery of the heart, causing damage to the heart muscle 1.

Choice B is incorrect because the aorta is not a blood vessel of the heart.
The aorta is the main artery that carries oxygenated blood from the heart to the rest of the body.
Choice C is incorrect because the pulmonary blood vessels are not affected by a myocardial infarction.
The pulmonary blood vessels carry deoxygenated blood from the heart to the lungs.
Choice D is incorrect because the vena cava is not a blood vessel of the heart.
The vena cava is a large vein that carries deoxygenated blood from the body to the heart.
Correct Answer is C
Explanation
The hypothalamus is a region of the brain that synthesizes antidiuretic hormone (ADH), also known as vasopressin.
ADH is then transported to the posterior pituitary gland via neurohypophysial capillaries, where it is stored until it is ready to be secreted into the circulation.

Choice A.
Pineal gland is not correct because it is a small endocrine gland located in the brain that secretes the hormone melatonin, which regulates sleep-wake cycles, but it does not synthesize ADH.
Choice B.
Thymus is not correct because it is a gland located in the chest that produces hormones involved in immune system development, but it does not synthesize ADH.
Choice D.
Pancreas is not correct because it is a gland located behind the stomach that secretes hormones such as insulin and glucagon, which regulate blood sugar levels, but it does not synthesize ADH.
Correct Answer is B
Explanation
Calcium ions play a crucial role in initiating muscle contraction.
When a muscle cell is stimulated to contract by an action potential, calcium channels open in the sarcoplasmic membrane and release calcium into the sarcoplasm.
Some of this calcium attaches to troponin, which causes it to change shape.
This shape change exposes binding sites for myosin on the actin filaments.
Myosin’s binding to actin causes crossbridge formation, and contraction of the muscle begins.

The other ions mentioned in the question do not have this specific role in muscle contraction.
Potassium ions are important for maintaining the resting membrane potential of cells, but they do not bind to the troponin complex.
Phosphorus ions are important for energy metabolism, but they do not bind to the troponin complex.
Sodium ions are important for generating action potentials, but they do not bind to the troponin complex.
Correct Answer is D
Explanation
Proteins.
Proteins are made up of amino acids which are organic molecules that contain both an amine functional group (–NH2) and a carboxylic acid functional group (– COOH).
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Choice A, Lipids, is not the correct answer because lipids are a group of naturally occurring molecules that include fats, waxes, sterols, fat-soluble vitamins, monoglycerides, diglycerides, triglycerides, phospholipids, and others.
They do not contain both an amine and carboxyl group.
Choice B, Chitin, is not the correct answer because chitin is a long-chain polymer of N-acetylglucosamine, a derivative of glucose.
It does not contain both an amine and carboxyl group.
Choice C, Cellulose, is not the correct answer because cellulose is an organic compound with the formula (C6H10O5)n, a polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units.
It does not contain both an amine and carboxyl group.
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