To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
Titration
Electrophoresis
Filtration
Spectrophotometry
Correct Answer : B
Electrophoresis is the most useful laboratory method for separating genomic DNA fragments by size.
Electrophoresis is a technique that uses an electric field to separate charged molecules, such as DNA fragments, based on their size and charge.
Choice A is not correct because titration is a laboratory method used to determine the concentration of a solution.
Choice C is not correct because filtration is a laboratory method used to separate solids from liquids.
Choice D is not correct because spectrophotometry is a laboratory method used to measure the absorbance of light by a solution.
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Related Questions
Correct Answer is C
Explanation
The hypothalamus is a region of the brain that synthesizes antidiuretic hormone (ADH), also known as vasopressin.
ADH is then transported to the posterior pituitary gland via neurohypophysial capillaries, where it is stored until it is ready to be secreted into the circulation.
Choice A.
Pineal gland is not correct because it is a small endocrine gland located in the brain that secretes the hormone melatonin, which regulates sleep-wake cycles, but it does not synthesize ADH.
Choice B.
Thymus is not correct because it is a gland located in the chest that produces hormones involved in immune system development, but it does not synthesize ADH.
Choice D.
Pancreas is not correct because it is a gland located behind the stomach that secretes hormones such as insulin and glucagon, which regulate blood sugar levels, but it does not synthesize ADH.
Correct Answer is C
Explanation
A tsunami is a catastrophic ocean wave that is usually caused by a submarine earthquake.
It can also be caused by an underwater or coastal landslide, the eruption of a volcano, or the impact of a meteor or comet in a body of water.
Choice A is not correct because sunspot activity does not cause tsunamis.
Choice B is not correct because lightning strikes do not cause tsunamis.
Choice D is not correct because flooding does not cause tsunamis.
Correct Answer is A
Explanation
As a solid turns to a liquid, the particles become less ordered and more free to move around.
Choice B is not correct because particles have an increase in mobility as a solid turns to a liquid.
Choice C is not correct because particles move further apart as a solid turns to a liquid.
Choice D is not correct because intermolecular forces between particles become weaker as a solid turns to a liquid.
Correct Answer is B
Explanation
The atomic number of an element represents the number of protons in the nucleus of an atom of that element.
Since lithium has an atomic number of 3, it has 3 protons in its nucleus.
Choice A is not correct because 7 is the mass number of lithium, not the number of protons.
Choice C is not correct because 12 is not the atomic number or mass number of lithium.
Choice D is not correct because 4 is not the atomic number or mass number of lithium.
Correct Answer is D
Explanation
The pleura is a double-layered serous membrane that covers each lung and lines the thoracic cage
The pleura is a vital part of the respiratory tract.
Its role is to cushion the lung and reduce any friction that may develop between the lung, rib cage, and chest cavity.
Each pleura (there are two) consists of a two-layered membrane that covers each lung.
The layers are separated by a small amount of viscous (thick) lubricant known as pleural fluid.
The pleura is comprised of two distinct layers: the visceral pleura and the parietal pleura.
The visceral pleura is the thin, slippery membrane that covers the surface of the lungs and dips into the areas separating the different lobes of the lungs (called the hilum).
Correct Answer is A
Explanation
The glomerulus is the main filtering unit of the kidney.
It is formed by a network of small blood vessels (capillaries) enclosed within a sac called the Bowman’s capsule.
The blood supply to the glomerulus is provided via the afferent arteriole.
The blood then flows through the capillary network, where it gets filtered, and then leaves the glomerulus via the efferent arteriole.
Choice B.
Proximal tubule is not correct because it is where the ultrafiltrate collected in the Bowman’s space drains directly into.
Choice C.
Distal tubule is not correct because it is not mentioned in relation to blood exiting the glomerulus.
Choice D.
Afferent arteriole is not correct because it provides blood supply to the glomerulus.
Correct Answer is C
Explanation
Heavier objects fall faster than lighter objects.
The data in the table shows that as the number of filters (and therefore the mass) increases, the terminal velocity also increases.
This means that the heavier objects (with more filters) are falling faster than the lighter objects (with fewer filters).
Choice A is not supported by the data in the table as much as it is true.
Terminal velocity is indeed when the force of gravity is equal to air resistance, but this definition does not provide any information about the relationship between mass and terminal velocity.
Choice B is also not supported by the data in the table.
The data does not provide any information about the time it takes for an object to reach terminal velocity.
Choice D is not supported by the data in the table.
The data shows that as the number of filters increases, the terminal velocity also increases.
This means that the greater the number of filters, the greater (not smaller) the terminal velocity.
Correct Answer is A
Explanation
Urea is a substance that is excreted by sweat glands in response to the breakdown of proteins and the formation of ammonia.
When proteins are broken down, they produce ammonia, which is a highly toxic compound for the body.
Ammonia is then converted into urea and released out of the body through sweat glands.
Choice B.
Sebum is not correct because it is an oily substance secreted by sebaceous glands to lubricate and protect the skin, but it is not related to the breakdown of proteins or the formation of ammonia.
Choice C.
Water is not correct because while it is a component of sweat, it is not specifically related to the breakdown of proteins or the formation of ammonia.
Choice D.
Lysozymes are not correct because they are enzymes found in tears, saliva and other body fluids that have antibacterial properties, but they are not related to the breakdown of proteins or the formation of ammonia.
Correct Answer is D
Explanation
Proteins.
Proteins are made up of amino acids which are organic molecules that contain both an amine functional group (–NH2) and a carboxylic acid functional group (– COOH).
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Choice A, Lipids, is not the correct answer because lipids are a group of naturally occurring molecules that include fats, waxes, sterols, fat-soluble vitamins, monoglycerides, diglycerides, triglycerides, phospholipids, and others.
They do not contain both an amine and carboxyl group.
Choice B, Chitin, is not the correct answer because chitin is a long-chain polymer of N-acetylglucosamine, a derivative of glucose.
It does not contain both an amine and carboxyl group.
Choice C, Cellulose, is not the correct answer because cellulose is an organic compound with the formula (C6H10O5)n, a polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units.
It does not contain both an amine and carboxyl group.
Correct Answer is B
Explanation
The hypothalamus is a small region of the brain located just above the brainstem. It serves as a critical control center for maintaining homeostasis — the body’s internal balance. One of its key roles is regulating core body temperature through a process called thermoregulation.
The hypothalamus monitors the body's temperature using temperature-sensitive neurons. If the body becomes too hot or too cold, the hypothalamus initiates responses to bring the temperature back to a normal range by:
- Triggering sweating to cool down the body.
- Initiating shivering to generate heat.
- Adjusting blood flow to the skin for heat loss or retention.
In addition to temperature regulation, the hypothalamus also controls other vital functions like:
- Hunger and thirst
- Sleep-wake cycles
- Hormone release (by influencing the pituitary gland)
- Stress response
Why the Other Options Are Incorrect:
- A. Pituitary gland: While the pituitary gland is known as the “master gland” because it controls other endocrine glands, it does so under the direction of the hypothalamus. It does not directly regulate body temperature.
- C. Adrenal gland: The adrenal glands produce hormones like cortisol and adrenaline, primarily involved in the stress response, not temperature regulation.
- D. Pancreas: The pancreas is mainly responsible for regulating blood sugar levels through insulin and glucagon, not body temperature.
Thus, the hypothalamus is the part of the endocrine system most responsible for maintaining homeostasis, including regulating core body temperature.
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