A gram negative organisms contain a thick peptidoglycan layer

Gram-negative organisms are characterized by a thin peptidoglycan layer located between the inner cytoplasmic membrane and an outer membrane containing lipopolysaccharide (LPS). The thin peptidoglycan layer is insufficient to retain the crystal violet stain during Gram staining, which is why gram-negative bacteria appear pink after counterstaining with safranin. In contrast, gram-positive bacteria have a thick peptidoglycan layer that retains the crystal violet-iodine complex, appearing purple under a microscope. The thin peptidoglycan and outer membrane structure of gram-negative bacteria also contributes to their increased resistance to certain antibiotics compared to gram-positive organisms

RNA viruses are categorized based on the structure and polarity of their RNA genome. Positive-sense RNA can act directly as messenger RNA and be translated by host ribosomes. Negative-sense RNA must first be transcribed into complementary mRNA by viral RNA-dependent RNA polymerase. Some RNA viruses possess double-stranded RNA genomes, which also require specialized replication mechanisms inside the host cell.