Gram-positive bacteria appear purple because they contain:
Thick peptidoglycan layer
Outer membrane
Lipopolysaccharide
The Correct Answer is A
A. Thick peptidoglycan layer: Gram-positive bacteria have a thick, multilayered peptidoglycan cell wall that retains the crystal violet-iodine complex during the Gram staining process. This dense layer prevents the dye from being washed out during the alcohol decolorization step, causing Gram-positive bacteria to appear purple under a microscope.
B. Outer membrane: The outer membrane is characteristic of Gram-negative bacteria, not Gram-positive bacteria. It contains lipopolysaccharides and provides an additional barrier but does not contribute to the retention of crystal violet in Gram-positive organisms.
C. Lipopolysaccharide: Lipopolysaccharides (LPS) are components of the outer membrane of Gram-negative bacteria and play a role in pathogenicity and immune activation. LPS is absent in Gram-positive bacteria and therefore does not influence their purple coloration during Gram staining.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
A. Periplasmic flagella: Spirochetes have flagella located within the periplasmic space between the inner and outer membranes. These internal flagella, also called axial filaments, rotate to produce a corkscrew-like motion, allowing the bacterium to move efficiently through viscous environments such as mucus or connective tissue. This unique mechanism is characteristic of genera like Treponema and Borrelia.
B. Pili: Pili are hair-like appendages on the surface of many bacteria used primarily for attachment to surfaces and for conjugation (gene transfer). Pili do not contribute to the corkscrew motility seen in spirochetes.
C. Capsules: Capsules are extracellular polysaccharide layers that protect bacteria from phagocytosis and desiccation. While important for virulence and immune evasion, capsules do not play a role in bacterial motility or the corkscrew movement of spirochetes.
Correct Answer is D
Explanation
A. log phase: The log phase, or exponential phase, is characterized by rapid, logarithmic growth of bacteria. During this period, the rate of cell division exceeds the rate of cell death, resulting in a steep increase in population size. Nutrients are abundant, and waste products have not yet accumulated to inhibitory levels.
B. lag phase: The lag phase is the initial period after bacteria are introduced into a new environment. During this phase, cells are metabolically active but not dividing, as they adapt to the new conditions, synthesize necessary enzymes, and prepare for active growth.
C. telophase: Telophase is a stage of eukaryotic mitosis, not a bacterial growth phase. Bacteria reproduce via binary fission, so this term does not apply to the bacterial growth curve.
D. stationary phase: The stationary phase occurs when nutrient depletion and waste accumulation slow microbial growth. During this phase, the rate of cell division equals the rate of cell death, resulting in a plateau in population size. This phase reflects environmental stress and triggers survival mechanisms in bacteria.
E. death phase: The death phase follows the stationary phase when the rate of cell death exceeds the rate of new cell formation. The bacterial population declines due to exhaustion of nutrients and accumulation of toxic byproducts.
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