Which of the following blood specimen tests are photosensitive and should be protected from light during handling and transportation?

Choice A reason:
Using a needle at a 45° angle does not typically result in collapsing the vein. A collapsed vein is more likely due to excessive vacuum pressure from the tube, a very small or fragile vein, or the tourniquet being too tight.

Choice B reason:
At a 45° angle, the needle is more likely to penetrate through the entire depth of the vein, especially if the vein is superficial. This can result in a failed venipuncture because the needle has entered and exited the vein, missing the opportunity to collect blood.

Choice C reason:
While a steep angle like 45° could cause the needle to miss the vein superficially, it is more likely that the needle would go through the vein at this angle rather than merely missing it superficially. A superficial miss is more likely with an angle that is too shallow.

Choice D reason:
Contacting the upper wall of the vein alone would not necessarily result in a failed venipuncture. However, at a 45° angle, the needle could easily slip through the vein entirely after contacting the upper wall, especially if the vein is not anchored well.

Choice A Reason:
Occult blood detection is a test that a phlebotomist is allowed to perform according to CLIA regulations. This test is typically used to detect hidden blood in the stool, which can be an early sign of colorectal cancer or other digestive system diseases. It is considered a waived test under CLIA, meaning it is simple and carries a low risk for an incorrect result.

Choice B Reason:
Flow cytometry is a complex test that requires specialized training and equipment. It is used to analyze the physical and chemical characteristics of particles in a fluid as they pass through at least one laser. Phlebotomists are not typically trained to perform this type of analysis, as it falls under the category of high complexity testing.

Choice C Reason:
Genetic molecular tests are used to detect specific sequences in DNA or RNA that may or may not lead to disease. These tests are complex and require specialized knowledge and equipment. They are not within the scope of practice for phlebotomists, who are trained primarily in blood collection.

Choice D Reason:
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge. This type of test is also complex and requires specialized training and equipment, which is beyond the scope of a phlebotomist's duties.