Which of the following blood specimen tests are photosensitive and should be protected from light during handling and transportation?

Choice A Reason:
Taking the specimen temperature before 4 minutes is the correct procedure according to the Department of Transportation (DOT) guidelines. The DOT Rule 49 CFR Part 40 Section 40.65 states that the temperature of the specimen must be checked no later than four minutes after the employee has given the specimen. The acceptable temperature range is 32–38 °C/90–100 °F, which helps to verify the validity of the specimen.

Choice B Reason:
Taking the specimen temperature at 5 minutes is not within the DOT guidelines. The temperature must be taken before 4 minutes have passed to ensure the specimen's integrity and to comply with the regulatory requirements.

Choice C Reason:
At 15 minutes, the temperature check would be too late. The DOT guidelines specify that the temperature should be checked no later than four minutes after collection to ensure the specimen has not been tampered with and is within the acceptable temperature range.

Choice D Reason:
After 30 minutes, the temperature of the urine specimen would not reflect the body temperature at the time of collection, which is necessary for the validity of the test. This delay could allow for the temperature to fall outside of the acceptable range, potentially invalidating the specimen.

Choice A reason:
The BUN test measures the amount of urea nitrogen in the blood, which can indicate kidney function. Fasting is not typically required for a BUN test unless it is being conducted alongside other tests that may require fasting.

Choice B reason:
An HDL test is part of a lipid profile that measures the levels of good cholesterol in the blood. Fasting before a cholesterol test, including HDL, is often recommended to ensure accuracy, although recent guidelines have changed, allowing for nonfasting cholesterol testing in certain individuals.

Choice C reason:
An ABG test measures oxygen and carbon dioxide levels in the blood and assesses the body's acid-base balance. Preparation for an ABG test does not typically require fasting, but patients may need to stop supplemental oxygen before the test to get accurate measurements.

Choice D reason:
A CBC test, or complete blood count, does not require fasting. It measures various factors of the blood, including red and white blood cells and platelets. Fasting is not necessary unless the CBC is part of a panel of tests that includes tests requiring fasting.