Which of the following tests requires heating prior to observing results?
Benedict's test
Brown paper test
Biuret test
Iodine test
Wendelspecht test
The Correct Answer is A
Choice A reason: Benedict's test is a test for the presence of reducing sugars, such as glucose or maltose, in a solution. The test involves adding Benedict's reagent, which is a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, to the solution and heating it in a water bath. If reducing sugars are present, they reduce the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. The color and amount of the precipitate indicate the concentration of reducing sugars in the solution. ¹
Choice B reason: Brown paper test is a test for the presence of lipids, such as fats or oils, in a solution. The test involves placing a drop of the solution on a piece of brown paper and letting it dry. If lipids are present, they leave a translucent spot on the paper, which can be seen by holding the paper against a light source. The test is based on the fact that lipids are nonpolar and do not dissolve in water, but can dissolve in organic solvents and stain the paper. ²
Choice C reason: Biuret test is a test for the presence of proteins or peptides in a solution. The test involves adding Biuret reagent, which is a blue solution of copper (II) sulfate and sodium hydroxide, to the solution. If proteins or peptides are present, they form a complex with the copper (II) ions, which changes the color of the solution to violet or pink. The test is based on the fact that proteins and peptides have peptide bonds, which have nitrogen atoms that can coordinate with the copper (II) ions. ³
Choice D reason: Iodine test is a test for the presence of starch in a solution. The test involves adding iodine solution, which is a brown solution of iodine and potassium iodide, to the solution. If starch is present, it forms a complex with the iodine molecules, which changes the color of the solution to blue-black. The test is based on the fact that starch is a polysaccharide that has a helical structure, which can trap the iodine molecules inside. ⁴
Choice E reason: Wendelspecht test is a fictional test that does not exist in reality. It is a made-up name that has no meaning or relevance to the topic of this question. Therefore, it cannot be a valid answer.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is D
Explanation
Choice A rationale: Only use lower power magnification is incorrect because this does not solve the problem of the dark field of view, but rather avoids it. Using lower power magnification means that you will not be able to see the details of the specimen that you want to observe under the 40x objective lens. Lower power magnification also has a larger field of view and a lower resolution than higher power magnification.
Choice B rationale: Add more immersion oil is incorrect because immersion oil is only used for the highest magnification objective lens, which is the 100x oil immersion objective lens, not the 40x objective lens. Immersion oil is a type of oil that has the same refractive index as glass, which means that it bends light in the same way as glass. Immersion oil is applied between the slide and the lens to reduce the refraction of light and increase the clarity of the image.
Choice C rationale: Adjust the fine adjustment knob is incorrect because the fine adjustment knob is used to fine-tune the focus of the specimen, not the brightness of the field of view. The fine adjustment knob is a small knob that is located on the side of the microscope, next to the coarse adjustment knob. The fine adjustment knob is used to make small changes in the distance between the objective lens and the specimen, which improves the sharpness of the image.
Choice D rationale: Increase the light intensity is correct because the light intensity is the amount of light that reaches the specimen and the objective lens, which affects the brightness of the field of view. The light intensity can be adjusted by using the rheostat, which is a dial that is located on the base of the microscope, next to the light source. The rheostat can increase or decrease the voltage of the light source, which changes the brightness of the light. Increasing the light intensity can make the field of view brighter and easier to see.
Correct Answer is A
Explanation
Choice A rationale: Carbon dioxide is not a product of the light reaction, but a reactant of the dark reaction. The light reaction does not use carbon dioxide, but water and light energy to produce ATP, NADPH, and oxygen¹.
Choice B rationale: NADPH is a product of the light reaction. It is an electron carrier that is reduced by the electrons from water in photosystem I. NADPH provides electrons and hydrogen for the dark reaction².
Choice C rationale: ATP is a product of the light reaction. It is an energy molecule that is synthesized by the enzyme ATP synthase using the proton gradient created by the electron transport chain. ATP provides energy for the dark reaction³.
Choice D rationale: Oxygen is a product of the light reaction. It is released as a by-product of the splitting of water in photosystem II. Oxygen is either used for cellular respiration or released into the atmosphere⁴.
Choice E rationale: Energy intermediates are not a specific product of the light reaction, but a general term for molecules that store energy or electrons, such as ATP and NADPH. Therefore, this choice is not incorrect, but less specific than choice A⁵.
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