Which reagent is used to detect the presence of protein?
Sudan IV
Benedict's
Biuret
Iodine
The Correct Answer is C
Choice A rationale: Sudan IV is not a reagent for protein detection, but a reagent for lipid detection. Sudan IV is a red dye that binds to non-polar molecules, such as fats and oils. Sudan IV stains lipids red, while leaving water-soluble molecules, such as proteins, unstained¹.
Choice B rationale: Benedict's is not a reagent for protein detection, but a reagent for sugar detection. Benedict's is a blue solution that contains copper sulfate, sodium carbonate, and sodium citrate. Benedict's reacts with reducing sugars, such as glucose and fructose, and reduces the copper ions from blue to orange-red².
Choice C rationale: Biuret is a reagent for protein detection. Biuret is a blue solution that contains copper sulfate and sodium hydroxide. Biuret reacts with peptide bonds, which are the bonds that link amino acids in proteins. Biuret changes color from blue to violet when it binds to protein molecules³.
Choice D rationale: Iodine is not a reagent for protein detection, but a reagent for starch detection. Iodine is a brown solution that forms a complex with starch, a polysaccharide composed of glucose units. Iodine changes color from brown to blue-black when it interacts with starch molecules⁴.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is D
Explanation
Choice A rationale: An isotonic solution has the same solute concentration as the plant cells, which means it has the same water potential. Water will move in and out of the plant cells at the same rate, causing them to remain unchanged in size and shape. This will not help the lettuce to become less wilted.
Choice B rationale: A hypertonic solution has a higher solute concentration than the plant cells, which means it has a lower water potential. Water will move out of the plant cells by osmosis, causing them to shrink and become plasmolysed. This will make the lettuce more wilted and not crisper.
Choice C rationale: Soaking the lettuce in water would have an effect, depending on the relative solute concentration of the water and the plant cells. Water will move across the cell membrane by osmosis, either into or out of the plant cells, causing them to change in size and shape.
Choice D rationale: A hypotonic solution has a lower solute concentration than the plant cells, which means it has a higher water potential. Water will move into the plant cells by osmosis, causing them to swell and become turgid. This will make the lettuce crisper and more appealing.
Choice E rationale: A hypotonic solution will cause the plant cells to gain water and become turgid, not more wilted. Wilted lettuce is caused by the loss of water from the plant cells, which makes them flaccid and soft.
Correct Answer is {"dropdown-group-1":"C"}
Explanation
Choice A rationale: Isotonic is incorrect because isotonic is a type of solution that has the same concentration of water and solute as the cell placed in the solution. In an isotonic solution, there is no net movement of water across the cell membrane.
Choice B rationale: Osmotic is incorrect because osmotic is an adjective that describes the movement of water across a semipermeable membrane, not a type of solution. Osmosis is the process by which water moves from a region of high water concentration to a region of low water concentration.
Choice C rationale: Hypotonic is correct because hypotonic is a type of solution that has a higher concentration of water and lower concentration of solute than the cell placed in the solution. In a hypotonic solution, water moves into the cell and out of the solution by osmosis, causing the cell to swell.
Choice D rationale: Diffusive is incorrect because diffusive is an adjective that describes the movement of molecules from a region of high concentration to a region of low concentration, not a type of solution. Diffusion is the process by which molecules move across a membrane or a space due to their random motion.
Choice E rationale: Hypertonic is incorrect because hypertonic is a type of solution that has a lower concentration of water and higher concentration of solute than the cell placed in the solution. In a hypertonic solution, water moves out of the cell and into the solution by osmosis, causing the cell to shrink.
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