Which of the following tests is used to detect the presence of lipids?
Biuret test
Gram's iodine test
Ninhydrin test
Brown paper test
Benedict's test
The Correct Answer is D
Choice A reason: Biuret test is a test for the presence of proteins or peptides in a solution. It involves adding Biuret reagent, which is a blue solution of copper (II) sulfate and sodium hydroxide, to the solution. If proteins or peptides are present, they form a complex with the copper (II) ions, which changes the color of the solution to violet or pink. ³
Choice B reason: Gram's iodine test is a test for the presence of starch in a solution. It involves adding iodine solution, which is a brown solution of iodine and potassium iodide, to the solution. If starch is present, it forms a complex with the iodine molecules, which changes the color of the solution to blue-black. ⁴
Choice C reason: Ninhydrin test is a test for the presence of amines or amino acids in a solution. It involves adding ninhydrin reagent, which is a purple solution of ninhydrin, to the solution. If amines or amino acids are present, they react with ninhydrin to produce a purple color, often called Ruhemann's purple. ¹
Choice D reason: Brown paper test is a test for the presence of lipids, such as fats or oils, in a solution. The test involves placing a drop of the solution on a piece of brown paper and letting it dry. If lipids are present, they leave a translucent spot on the paper, which can be seen by holding the paper against a light source. The test is based on the fact that lipids are nonpolar and do not dissolve in water, but can dissolve in organic solvents and stain the paper. ²
Choice E reason: Benedict's test is a test for the presence of reducing sugars, such as glucose or maltose, in a solution. The test involves adding Benedict's reagent, which is a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, to the solution and heating it in a water bath. If reducing sugars are present, they reduce the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. ⁵
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is ["A","B","E","F"]
Explanation
Choice A rationale: Transfer pipette is correct because you need a transfer pipette to transfer a drop of water or a liquid specimen onto the center of the slide. A transfer pipette is a plastic or glass tube with a bulb at one end that can suck up and release liquids.
Choice B rationale: Clean slide is correct because you need a clean slide to place the specimen on. A slide is a thin, flat piece of glass or plastic that can be viewed under a microscope. A clean slide ensures that there is no contamination or interference from other substances.
Choice C rationale: Bunsen burner is incorrect because you do not need a Bunsen burner to make a wet mount. A Bunsen burner is a device that produces a flame by mixing gas and air. A Bunsen burner is used for heating, sterilizing, or burning substances, not for making a wet mount.
Choice D rationale: Timer is incorrect because you do not need a timer to make a wet mount. A timer is a device that measures or indicates the passage of time. A timer is used for timing experiments, not for making a wet mount.
Choice E rationale: Tweezers is correct because you need tweezers to pick up and place the specimen on the slide. Tweezers are a pair of metal or plastic pincers that can grasp and hold small objects. Tweezers are useful for handling delicate or microscopic specimens.
Choice F rationale: Cover slip is correct because you need a cover slip to cover the specimen on the slide. A cover slip is a thin, transparent piece of glass or plastic that can be placed over the specimen on the slide. A cover slip protects the specimen from drying out, dust, or damage, and creates a flat surface for viewing under the microscope.
Correct Answer is B
Explanation
The total magnification of a microscope is calculated by multiplying the magnification of the objective lens and the magnification of the eyepiece lens. Therefore, using a 10× objective lens with a 10× eyepiece lens would result in a total magnification of 10×10 = 100×, not 20×.
To achieve a total magnification of 20×, one would need to use a 2× objective lens with a 10× eyepiece lens, or a 10× objective lens with a 2× eyepiece lens. Therefore, the statement that the total magnification achieved using a 10× objective lens with a 10× eyepiece lens is 20× is false.
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