A phlebotomist has collected a lactic acid sample and needs to prepare the sample for processing and transportation. Which of the following methods is correct?

Choice A Reason:
The patient's condition is a critical factor as it can influence the ease of blood collection and the choice of collection site. For example, a dehydrated patient may have less accessible veins. Patient age is also important; pediatric and geriatric patients may require different approaches due to vein size and fragility. The blood volume required for testing must be considered to ensure sufficient sample size without compromising patient safety, adhering to guidelines that limit blood collection to a certain percentage of the patient's total blood volume.

Choice B Reason:
While patient height and gender may influence the choice of blood collection site, they are not as critical as the factors listed in choice A. Patient medications are important to consider, as some medications may affect coagulation or the integrity of the sample.

Choice C Reason:
The ordering physician's preferences may be considered, but they are not a primary factor in the method of collection. The number of tests ordered and the diagnosis can influence the volume of blood needed but are secondary to the patient's condition and safety considerations.

Choice D Reason:
The time of day and the timing of the specimen collection can be important for certain tests that are time-sensitive or influenced by diurnal variations. The test status, whether routine or stat, may affect the urgency of collection but not necessarily the method of collection.

Choice A Reason:
The red top tube is typically used for serum determinations in chemistry, serology, and blood bank. It contains no additive or may contain a clot activator. The red top tube is not the first choice for capillary puncture order of draw because it is designed for tests that require serum and not whole blood.

Choice B Reason:
The lavender top tube is used for whole blood hematology determinations, as it contains EDTA as an anticoagulant. According to the order of draw for capillary punctures, the EDTA tube should be collected first to ensure adequate volume and accurate hematology test results. This makes the lavender top tube the correct choice for the first collection in this scenario.

Choice C Reason:
The light blue top tube contains sodium citrate and is used for coagulation tests. In venipuncture, it is drawn after the blood culture bottle and before other tubes to prevent contamination with additives from other tubes. However, for capillary punctures, it is not the first choice as hematology tests take precedence.

Choice D Reason:
The royal blue top tube is used for trace element, toxicology, and nutrient determinations². While it may be used for lead and toxicology screens mentioned in the question, it is not the first tube to be drawn in a capillary puncture sequence. The EDTA tube (lavender top) is drawn first to prevent clotting and ensure accurate CBC results.