A phlebotomist should instruct a patient to take which of the following actions when providing a sputum specimen?

Choice A reason:
A specimen bag is typically used for transporting specimens from the collection site to the laboratory. It is not designed for the disposal of contaminated items such as microscope slides. These bags are not puncture-resistant and do not provide the necessary protection against sharps injuries or contamination.

Choice B reason:
A biohazard bag is used for disposing of items that have been contaminated with potentially infectious materials. While it is suitable for materials like gloves, tissues, or any other items that do not have sharp edges, it is not the appropriate choice for glass slides, which can puncture the bag and pose a risk to handlers.

Choice C reason:
A red sharps container is specifically designed for the disposal of sharps waste, which includes any device that can puncture or cut skin, such as needles, syringes, lancets, and contaminated glass such as microscope slides. These containers are rigid, puncture-resistant, leak-proof, and usually marked with a biohazard symbol, making them the correct choice for disposing of contaminated microscope slides.

Choice D reason:
A medical waste container is a broad term that can refer to various types of containers used for medical waste. However, not all medical waste containers are suitable for sharps disposal. Unless the medical waste container is designed like a red sharps container, it may not be safe for disposing of glass slides.

Choice A Reason:
Body temperature does not significantly affect serum cortisol levels and is not a factor that needs to be considered when planning to collect a cortisol specimen. Cortisol levels are regulated by the hypothalamic-pituitary-adrenal axis and not by body temperature.

Choice B Reason:
Time of day is a critical factor to consider when collecting a specimen for serum cortisol level measurement. Cortisol levels follow a diurnal pattern, peaking in the early morning hours and declining throughout the day, with the lowest levels typically occurring around midnight¹². This variation is known as the cortisol awakening response (CAR) and is important for accurately interpreting the results of the test.

Choice C Reason:
Alcohol consumption can affect cortisol levels; however, it is not typically a factor that a phlebotomist needs to consider immediately before specimen collection unless the patient is under the influence at the time of the test. Chronic alcohol use can lead to alterations in cortisol levels, but this is more of a consideration for the healthcare provider interpreting the results rather than the phlebotomist collecting the specimen.

Choice D Reason:
Prandial status, or the fed or fasting state of the patient, can influence cortisol levels, but it is not as significant a factor as the time of day. Cortisol levels can be slightly higher after eating, but this effect is generally considered minimal. The primary concern for prandial status would be if the cortisol test is being conducted alongside other tests that require fasting.