The negative control used in the tests for protein, lipids, sugars, and starch was

Choice A rationale: Albumin solution is not a negative control, but a positive control for the test for protein. Albumin is a type of protein that reacts with the biuret reagent and produces a violet color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.

Choice B rationale: Starch solution is not a negative control, but a positive control for the test for starch. Starch is a type of carbohydrate that reacts with the iodine solution and produces a blue-black color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.

Choice C rationale: Glucose solution is not a negative control, but a positive control for the test for sugar. Glucose is a type of sugar that reacts with the Benedict's solution and produces a red-orange color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.

Choice D rationale: Olive oil is not a negative control, but a positive control for the test for lipids. Olive oil is a type of lipid that reacts with the Sudan III solution and produces a red color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.

Choice E rationale: Distilled water is a negative control for the tests for protein, lipids, sugars, and starch. Distilled water is a pure solvent that does not contain any of these substances. It does not react with any of the reagents and does not produce any color change. A negative control is used to confirm that there is no response to the reagent or the microorganism used in the test. It is used to set the baseline and verify that the detecting reagent is working properly³.

Choice A rationale: Only use lower power magnification is incorrect because this does not solve the problem of the dark field of view, but rather avoids it. Using lower power magnification means that you will not be able to see the details of the specimen that you want to observe under the 40x objective lens. Lower power magnification also has a larger field of view and a lower resolution than higher power magnification.

Choice B rationale: Add more immersion oil is incorrect because immersion oil is only used for the highest magnification objective lens, which is the 100x oil immersion objective lens, not the 40x objective lens. Immersion oil is a type of oil that has the same refractive index as glass, which means that it bends light in the same way as glass. Immersion oil is applied between the slide and the lens to reduce the refraction of light and increase the clarity of the image.

Choice C rationale: Adjust the fine adjustment knob is incorrect because the fine adjustment knob is used to fine-tune the focus of the specimen, not the brightness of the field of view. The fine adjustment knob is a small knob that is located on the side of the microscope, next to the coarse adjustment knob. The fine adjustment knob is used to make small changes in the distance between the objective lens and the specimen, which improves the sharpness of the image.

Choice D rationale: Increase the light intensity is correct because the light intensity is the amount of light that reaches the specimen and the objective lens, which affects the brightness of the field of view. The light intensity can be adjusted by using the rheostat, which is a dial that is located on the base of the microscope, next to the light source. The rheostat can increase or decrease the voltage of the light source, which changes the brightness of the light. Increasing the light intensity can make the field of view brighter and easier to see.