Which reagent is used to detect the presence of starch?
Iodine
Biuret
Benedict's
Phenol red
Sudan IV
The Correct Answer is A
Choice A rationale: Iodine is a chemical element that forms a brown solution of iodine and potassium iodide, known as iodine solution. When this solution is added to a sample that contains starch, it forms a complex with the starch molecules, which changes the color of the solution to blue-black. This is based on the fact that starch is a polysaccharide that has a helical structure, which can trap the iodine molecules inside. ²
Choice B rationale: Biuret is a chemical compound that forms a blue solution of copper (II) sulfate and sodium hydroxide, known as biuret reagent. When this reagent is added to a sample that contains proteins or peptides, it forms a complex with the copper (II) ions, which changes the color of the solution to violet or pink. This is based on the fact that proteins and peptides have peptide bonds, which have nitrogen atoms that can coordinate with the copper (II) ions. ³
Choice C rationale: Benedict's is a chemical compound that forms a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, known as Benedict's reagent. When this reagent is heated with a sample that contains reducing sugars, such as glucose or maltose, it reduces the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. This is based on the fact that reducing sugars have free aldehyde or ketone groups that can donate electrons to the copper (II) ions. ⁴
Choice D rationale: Phenol red is a chemical compound that forms a red solution that is used as a pH indicator. When this solution is added to a sample that has an acidic or neutral pH, it remains red or turns yellow. When this solution is added to a sample that has an alkaline pH, it turns pink or fuchsia. This is based on the fact that phenol red has a sulfonated hydroxyquinone group that can lose or gain protons depending on the pH of the solution. ⁵
Choice E rationale: Sudan IV is a chemical compound that forms a red powder that is used as a stain for lipids. When this powder is dissolved in a solvent and added to a sample that contains lipids, such as fats or oils, it dissolves in the lipids and stains them red. When this solution is added to a sample that does not contain lipids, it remains in the solvent and does not stain the sample. This is based on the fact that Sudan IV is a nonpolar compound that can dissolve in nonpolar substances like lipids, but not in polar substances like water. ⁶.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is D
Explanation
Choice A rationale: Locates the specimen is incorrect because the fine adjustment knob does not help to find the specimen on the slide, but rather to adjust the focus of the specimen. The fine adjustment knob is a small knob that is located on the side of the microscope, next to the coarse adjustment knob. The fine adjustment knob is used to make small changes in the distance between the objective lens and the specimen, which improves the sharpness of the image.
Choice B rationale: All of the answer choices are correct is incorrect because only one of the answer choices is correct. The fine adjustment knob only fine-tunes the focus of the specimen, not locates the specimen or the focus plane.
Choice C rationale: Locates the focus plane is incorrect because the fine adjustment knob does not help to find the focus plane, but rather to adjust the focus of the specimen. The focus plane is the plane that is perpendicular to the optical axis of the microscope and passes through the focal point of the objective lens. The focus plane is where the specimen is in focus and the image is clear.
Choice D rationale: Fine-tunes the focus is correct because the fine adjustment knob is used to fine-tune the focus of the specimen, which means to make small adjustments in the focus to obtain the best image quality. The fine adjustment knob is used after the coarse adjustment knob, which is used to bring the specimen into approximate focus.
Correct Answer is A
Explanation
Diffusion is a passive process that does not require energy input from the cell. It is driven by the random motion of molecules that results in a net movement from regions of higher concentration to regions of lower concentration.
Diffusion is one of the ways that substances can cross the cell membrane, such as oxygen, carbon dioxide, and water. Diffusion is important for cellular functions, such as gas exchange, nutrient uptake, and waste removal. Diffusion also helps maintain the homeostasis of the cell by balancing the concentration gradients of different molecules. Therefore, diffusion is the movement of a molecule from an area of higher concentration to an area of lower concentration.
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