Which reagent is used to detect the presence of starch?
Iodine
Biuret
Benedict's
Phenol red
Sudan IV
The Correct Answer is A
Choice A rationale: Iodine is a chemical element that forms a brown solution of iodine and potassium iodide, known as iodine solution. When this solution is added to a sample that contains starch, it forms a complex with the starch molecules, which changes the color of the solution to blue-black. This is based on the fact that starch is a polysaccharide that has a helical structure, which can trap the iodine molecules inside. ²
Choice B rationale: Biuret is a chemical compound that forms a blue solution of copper (II) sulfate and sodium hydroxide, known as biuret reagent. When this reagent is added to a sample that contains proteins or peptides, it forms a complex with the copper (II) ions, which changes the color of the solution to violet or pink. This is based on the fact that proteins and peptides have peptide bonds, which have nitrogen atoms that can coordinate with the copper (II) ions. ³
Choice C rationale: Benedict's is a chemical compound that forms a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, known as Benedict's reagent. When this reagent is heated with a sample that contains reducing sugars, such as glucose or maltose, it reduces the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. This is based on the fact that reducing sugars have free aldehyde or ketone groups that can donate electrons to the copper (II) ions. ⁴
Choice D rationale: Phenol red is a chemical compound that forms a red solution that is used as a pH indicator. When this solution is added to a sample that has an acidic or neutral pH, it remains red or turns yellow. When this solution is added to a sample that has an alkaline pH, it turns pink or fuchsia. This is based on the fact that phenol red has a sulfonated hydroxyquinone group that can lose or gain protons depending on the pH of the solution. ⁵
Choice E rationale: Sudan IV is a chemical compound that forms a red powder that is used as a stain for lipids. When this powder is dissolved in a solvent and added to a sample that contains lipids, such as fats or oils, it dissolves in the lipids and stains them red. When this solution is added to a sample that does not contain lipids, it remains in the solvent and does not stain the sample. This is based on the fact that Sudan IV is a nonpolar compound that can dissolve in nonpolar substances like lipids, but not in polar substances like water. ⁶.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is E
Explanation
Choice A rationale: Albumin solution is not a negative control, but a positive control for the test for protein. Albumin is a type of protein that reacts with the biuret reagent and produces a violet color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.
Choice B rationale: Starch solution is not a negative control, but a positive control for the test for starch. Starch is a type of carbohydrate that reacts with the iodine solution and produces a blue-black color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.
Choice C rationale: Glucose solution is not a negative control, but a positive control for the test for sugar. Glucose is a type of sugar that reacts with the Benedict's solution and produces a red-orange color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.
Choice D rationale: Olive oil is not a negative control, but a positive control for the test for lipids. Olive oil is a type of lipid that reacts with the Sudan III solution and produces a red color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.
Choice E rationale: Distilled water is a negative control for the tests for protein, lipids, sugars, and starch. Distilled water is a pure solvent that does not contain any of these substances. It does not react with any of the reagents and does not produce any color change. A negative control is used to confirm that there is no response to the reagent or the microorganism used in the test. It is used to set the baseline and verify that the detecting reagent is working properly³.
Correct Answer is C
Explanation
Choice A rationale: Eukaryotes is incorrect because eukaryotes are organisms that have cells with a nucleus and other membrane-bound organelles, such as animals, plants, fungi, and protists. Some eukaryotes, such as protists, are microscopic and require microscopy to be studied, but others, such as animals and plants, are macroscopic and can be seen with the naked eye.
Choice B rationale: Fungi is incorrect because fungi are a group of eukaryotic organisms that include mushrooms, molds, yeasts, and lichens. Some fungi, such as yeasts and molds, are microscopic and require microscopy to be studied, but others, such as mushrooms and lichens, are macroscopic and can be seen with the naked eye.
Choice C rationale: Prokaryotes is correct because prokaryotes are organisms that have cells without a nucleus or other membrane-bound organelles, such as bacteria and archaea. All prokaryotes are microscopic and require microscopy to be studied. Prokaryotes are the smallest and most abundant living organisms on Earth.
Choice D rationale: Plants is incorrect because plants are a group of eukaryotic organisms that have cells with a cell wall, chloroplasts, and a large central vacuole, such as mosses, ferns, and flowering plants. Some plants, such as mosses and algae, are microscopic and require microscopy to be studied, but others, such as ferns and flowering plants, are macroscopic and can be seen with the naked eye.
Whether you are a student looking to ace your exams or a practicing nurse seeking to enhance your expertise , our nursing education contents will empower you with the confidence and competence to make a difference in the lives of patients and become a respected leader in the healthcare field.
Visit Naxlex, invest in your future and unlock endless possibilities with our unparalleled nursing education contents today
Report Wrong Answer on the Current Question
Do you disagree with the answer? If yes, what is your expected answer? Explain.
Kindly be descriptive with the issue you are facing.