A phlebotomist is performing a venipuncture on a patient who is allergic to alcohol. Which of the following is an appropriate antiseptic to use in place of alcohol?

Choice A reason:
The BUN test measures the amount of urea nitrogen in the blood, which can indicate kidney function. Fasting is not typically required for a BUN test unless it is being conducted alongside other tests that may require fasting.

Choice B reason:
An HDL test is part of a lipid profile that measures the levels of good cholesterol in the blood. Fasting before a cholesterol test, including HDL, is often recommended to ensure accuracy, although recent guidelines have changed, allowing for nonfasting cholesterol testing in certain individuals.

Choice C reason:
An ABG test measures oxygen and carbon dioxide levels in the blood and assesses the body's acid-base balance. Preparation for an ABG test does not typically require fasting, but patients may need to stop supplemental oxygen before the test to get accurate measurements.

Choice D reason:
A CBC test, or complete blood count, does not require fasting. It measures various factors of the blood, including red and white blood cells and platelets. Fasting is not necessary unless the CBC is part of a panel of tests that includes tests requiring fasting.

Choice A reason:
Inverting the tube 5 to 8 times ensures that the clot activator mixes thoroughly with the blood, promoting proper clotting. Setting the tube aside for 30 minutes allows sufficient time for the blood to clot before centrifugation. This is the recommended procedure to ensure that the serum is properly separated from the clot, resulting in a quality specimen suitable for testing.

Choice B reason:
Inverting the tube only 1 to 3 times may not be enough to mix the clot activator with the blood adequately, which could lead to incomplete clotting and potentially compromised test results. Additionally, setting the tube for 1 hour before centrifugation is longer than necessary and does not offer any advantage over the recommended 30 minutes.

Choice C reason:
Inverting the tube 2 to 4 times might not fully mix the clot activator with the blood, and setting it for only 15 minutes does not provide enough time for proper clot formation. This could result in a suboptimal separation of serum and clot, affecting the integrity of the test results.

Choice D reason:
Inverting the tube 8 to 10 times could potentially cause hemolysis or disruption of the blood cells due to excessive agitation. Setting the tube for 2 hours prior to centrifugation is not recommended as it could lead to serum degradation or other changes that may affect the test results.