Place the following steps in order:
Inoculate
Identify
Isolate
Incubate
Inspect
The Correct Answer is A,D,C,E,B
A) Inoculate — This is the first step in culturing microorganisms. The specimen or sample is introduced (inoculated) into a growth medium (solid or liquid) under sterile conditions to encourage microbial growth.
D) Incubate — After inoculation, the culture is placed in an incubator under suitable conditions of temperature, humidity, and atmosphere to allow growth.
C) Isolate — Once growth is visible, specific colonies are separated (isolated) to obtain pure cultures. This allows studying a single species of microorganism.
E) Inspect — The isolated colonies are then visually inspected (using naked eye or microscope) for morphological characteristics such as shape, color, size, and pattern.
B) Identify — Finally, biochemical, molecular, or serological tests are performed to identify the organism based on its characteristics.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is D
Explanation
A. rRNA only: Some genes encode ribosomal RNA, but not all genes are limited to rRNA.
B. tRNA only: Some genes encode transfer RNA, but genes can encode other RNA types or proteins as well.
C. mRNA and polypeptide only: Some genes encode mRNA that is translated into polypeptides, but genes also encode functional RNAs that are not translated.
D. Any of the above: A gene can encode rRNA, tRNA, or mRNA (which may be translated into a polypeptide), so any of these products can be specified by different genes.
Correct Answer is B
Explanation
A. Apoenzymes: Apoenzymes are the protein portion of enzymes and do not indicate whether the enzyme is secreted or extracellular.
B. Exoenzymes: Exoenzymes are secreted by the cell into the surrounding medium to hydrolyze large extracellular substrates like casein, producing clear zones around colonies.
C. Ribozymes: Ribozymes are RNA molecules with catalytic activity that function mainly inside cells; they are not secreted protein enzymes that digest extracellular proteins like casein.
D. Regulated enzymes: The enzymes degrading casein may be regulated, but the defining feature shown (activity outside the colony) is secretion, so regulated describes control rather than location of action.
E. Endoenzymes: Endoenzymes act inside the cell; enzymes that produce extracellular clearing of casein are acting outside the cell and therefore are not endoenzymes.
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