Place the following steps in order:
Inoculate
Identify
Isolate
Incubate
Inspect
The Correct Answer is A,D,C,E,B
A) Inoculate — This is the first step in culturing microorganisms. The specimen or sample is introduced (inoculated) into a growth medium (solid or liquid) under sterile conditions to encourage microbial growth.
D) Incubate — After inoculation, the culture is placed in an incubator under suitable conditions of temperature, humidity, and atmosphere to allow growth.
C) Isolate — Once growth is visible, specific colonies are separated (isolated) to obtain pure cultures. This allows studying a single species of microorganism.
E) Inspect — The isolated colonies are then visually inspected (using naked eye or microscope) for morphological characteristics such as shape, color, size, and pattern.
B) Identify — Finally, biochemical, molecular, or serological tests are performed to identify the organism based on its characteristics.
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Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is B
Explanation
A. DNA fingerprinting: DNA fingerprinting is mainly used for identification and forensics, and it generally faces minimal ethical opposition.
B. Modification of food crops: Genetic modification of crops raises public and ethical concerns related to environmental impact, safety, and food labeling.
C. Correcting gene defects in animals: Gene therapy in animals has fewer controversies than food crop modification, though it raises welfare concerns.
D. Sequencing of the human genome: Sequencing the human genome itself has faced limited ethical opposition; concerns are more focused on how the data are used, not the sequencing process.
Correct Answer is D
Explanation
A. Are acellular: Subunit vaccines contain only parts of the pathogen, not the whole cell or organism, which reduces the risk of adverse reactions.
B. Are administered in food: Vaccines are generally not delivered through food; this does not contribute to their safety.
C. Do not pose a risk for causing the disease: Because they contain only specific antigens and no live pathogen, subunit vaccines cannot revert to a virulent form or cause infection.
D. Are acellular and do not pose a risk for causing the disease: Subunit vaccines are acellular and composed of purified antigens, making them both safer and incapable of causing disease.
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