The biuret test would indicate a positive result for proteins in which of the following solutions?
Starch solution
Olive oil
Albumin solution
Distilled water
Glucose solution
The Correct Answer is C
Choice A rationale: Starch solution is not a protein solution, but a carbohydrate solution. Starch is a polysaccharide, which is a polymer of glucose molecules. Starch does not contain peptide bonds, which are the bonds that link amino acids in proteins. Therefore, starch solution would not react with the biuret reagent and would not produce a violet color.
Choice B rationale: Olive oil is not a protein solution, but a lipid solution. Olive oil is mainly composed of triglycerides, which are esters of glycerol and fatty acids. Olive oil does not contain peptide bonds, which are the bonds that link amino acids in proteins. Therefore, olive oil would not react with the biuret reagent and would not produce a violet color.
Choice C rationale: Albumin solution is a protein solution. Albumin is a globular protein that is found in blood plasma and egg white. Albumin contains many peptide bonds, which are the bonds that link amino acids in proteins. Therefore, albumin solution would react with the biuret reagent and would produce a violet color.
Choice D rationale: Distilled water is not a protein solution, but a pure solvent. Distilled water is water that has been purified by boiling and condensing. Distilled water does not contain any solutes, such as proteins, carbohydrates, or lipids. Therefore, distilled water would not react with the biuret reagent and would not produce a violet color.
Choice E rationale: Glucose solution is not a protein solution, but a carbohydrate solution. Glucose is a monosaccharide, which is a simple sugar. Glucose does not contain peptide bonds, which are the bonds that link amino acids in proteins. Therefore, glucose solution would not react with the biuret reagent and would not produce a violet color.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is D
Explanation
Choice A rationale: 4x is incorrect because 4x is the lowest magnification objective lens that does not require oil to be applied. 4x is also called the scanning objective lens because it is used to scan the whole slide and find the specimen. 4x has a large field of view and a low resolution.
Choice B rationale: 10x is incorrect because 10x is the medium magnification objective lens that does not require oil to be applied. 10x is also called the low power objective lens because it is used to observe the specimen in more detail. 10x has a smaller field of view and a higher resolution than 4x.
Choice C rationale: 40x is incorrect because 40x is the high magnification objective lens that does not require oil to be applied. 40x is also called the high power objective lens because it is used to observe the specimen in greater detail. 40x has a smaller field of view and a higher resolution than 10x.
Choice D rationale: 100x is correct because 100x is the highest magnification objective lens that requires oil to be applied. 100x is also called the oil immersion objective lens because it is used to observe the specimen in the highest detail. 100x has a very small field of view and a very high resolution. Oil is applied between the slide and the lens to reduce the refraction of light and increase the clarity of the image.
Correct Answer is B
Explanation
Choice A rationale: High power is incorrect because high power is the second highest magnification objective lens, not the highest. High power is also called the 40x objective lens because it magnifies the specimen by 40 times. When combined with the 10x eyepiece lens, the total magnification is 400x.
Choice B rationale: Oil immersion is correct because oil immersion is the highest magnification objective lens. Oil immersion is also called the 100x objective lens because it magnifies the specimen by 100 times. When combined with the 10x eyepiece lens, the total magnification is 1000x. Oil immersion requires oil to be applied between the slide and the lens to reduce the refraction of light and increase the clarity of the image.
Choice C rationale: Low power is incorrect because low power is the second lowest magnification objective lens, not the highest. Low power is also called the 10x objective lens because it magnifies the specimen by 10 times. When combined with the 10x eyepiece lens, the total magnification is 100x.
Choice D rationale: Scanning is incorrect because scanning is the lowest magnification objective lens, not the highest. Scanning is also called the 4x objective lens because it magnifies the specimen by 4 times. When combined with the 10x eyepiece lens, the total magnification is 40x. Scanning is used to scan the whole slide and find the specimen.
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