Which of the following items will you need to make a wet mount?
Check all that apply.
Transfer pipette
Clean slide
Bunsen burner
Timer
Cover slip
Correct Answer : A,B,E,F
Choice A rationale: Transfer pipette is correct because you need a transfer pipette to transfer a drop of water or a liquid specimen onto the center of the slide. A transfer pipette is a plastic or glass tube with a bulb at one end that can suck up and release liquids.
Choice B rationale: Clean slide is correct because you need a clean slide to place the specimen on. A slide is a thin, flat piece of glass or plastic that can be viewed under a microscope. A clean slide ensures that there is no contamination or interference from other substances.
Choice C rationale: Bunsen burner is incorrect because you do not need a Bunsen burner to make a wet mount. A Bunsen burner is a device that produces a flame by mixing gas and air. A Bunsen burner is used for heating, sterilizing, or burning substances, not for making a wet mount.
Choice D rationale: Timer is incorrect because you do not need a timer to make a wet mount. A timer is a device that measures or indicates the passage of time. A timer is used for timing experiments, not for making a wet mount.
Choice E rationale: Tweezers is correct because you need tweezers to pick up and place the specimen on the slide. Tweezers are a pair of metal or plastic pincers that can grasp and hold small objects. Tweezers are useful for handling delicate or microscopic specimens.
Choice F rationale: Cover slip is correct because you need a cover slip to cover the specimen on the slide. A cover slip is a thin, transparent piece of glass or plastic that can be placed over the specimen on the slide. A cover slip protects the specimen from drying out, dust, or damage, and creates a flat surface for viewing under the microscope.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is C
Explanation
Choice A rationale: Starch solution is not a protein solution, but a carbohydrate solution. Starch is a polysaccharide, which is a polymer of glucose molecules. Starch does not contain peptide bonds, which are the bonds that link amino acids in proteins. Therefore, starch solution would not react with the biuret reagent and would not produce a violet color.
Choice B rationale: Olive oil is not a protein solution, but a lipid solution. Olive oil is mainly composed of triglycerides, which are esters of glycerol and fatty acids. Olive oil does not contain peptide bonds, which are the bonds that link amino acids in proteins. Therefore, olive oil would not react with the biuret reagent and would not produce a violet color.
Choice C rationale: Albumin solution is a protein solution. Albumin is a globular protein that is found in blood plasma and egg white. Albumin contains many peptide bonds, which are the bonds that link amino acids in proteins. Therefore, albumin solution would react with the biuret reagent and would produce a violet color.
Choice D rationale: Distilled water is not a protein solution, but a pure solvent. Distilled water is water that has been purified by boiling and condensing. Distilled water does not contain any solutes, such as proteins, carbohydrates, or lipids. Therefore, distilled water would not react with the biuret reagent and would not produce a violet color.
Choice E rationale: Glucose solution is not a protein solution, but a carbohydrate solution. Glucose is a monosaccharide, which is a simple sugar. Glucose does not contain peptide bonds, which are the bonds that link amino acids in proteins. Therefore, glucose solution would not react with the biuret reagent and would not produce a violet color.
Correct Answer is B
Explanation
Choice A rationale: Sudan IV is a stain used to stain lipids. It is a red powder that dissolves in lipids and stains them red, but does not react with sugars. ¹
Choice B rationale: Benedict's is a reagent used to test for reducing sugars. It is a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate that reduces the copper (II) ions to copper (I) ions when heated with a reducing sugar, forming a red, orange, or green precipitate of copper (I) oxide. ⁴
Choice C rationale: Biuret is a reagent used to test for proteins and polypeptides. It is a blue solution of copper (II) sulfate and sodium hydroxide that forms a violet or pink complex with the peptide bonds in proteins or peptides. ⁸
Choice D rationale: Phenol red is a pH indicator. It is a red solution that changes color from yellow to red over the pH range 6.8 to 8.2, and from pink to fuchsia over 8.2 to 10.0. It does not react with sugars. ¹¹
Choice E rationale: Iodine is a reagent used to test for starch. It is a brown solution of iodine and potassium iodide that forms a blue-black complex with the starch molecules. It does not react with simple sugars. ¹⁴
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