When a serum separation tube (SST) is collected, which of the following actions should the phlebotomist take to ensure optimal test results?
Invert the tube 5 to 8 times and then set for 30 minutes prior to centrifugation.
Invert the tube 1 to 3 times and then set for 1 hour prior to centrifugation.
Invert the tube 2 to 4 times and then set for 15 minutes prior to centrifugation.
Invert the tube 8 to 10 times and then set for 2 hours prior to centrifugation.
The Correct Answer is A
Choice A reason:
Inverting the tube 5 to 8 times ensures that the clot activator mixes thoroughly with the blood, promoting proper clotting. Setting the tube aside for 30 minutes allows sufficient time for the blood to clot before centrifugation. This is the recommended procedure to ensure that the serum is properly separated from the clot, resulting in a quality specimen suitable for testing.
Choice B reason:
Inverting the tube only 1 to 3 times may not be enough to mix the clot activator with the blood adequately, which could lead to incomplete clotting and potentially compromised test results. Additionally, setting the tube for 1 hour before centrifugation is longer than necessary and does not offer any advantage over the recommended 30 minutes.
Choice C reason:
Inverting the tube 2 to 4 times might not fully mix the clot activator with the blood, and setting it for only 15 minutes does not provide enough time for proper clot formation. This could result in a suboptimal separation of serum and clot, affecting the integrity of the test results.
Choice D reason:
Inverting the tube 8 to 10 times could potentially cause hemolysis or disruption of the blood cells due to excessive agitation. Setting the tube for 2 hours prior to centrifugation is not recommended as it could lead to serum degradation or other changes that may affect the test results.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is B
Explanation
Choice A reason:
The date of birth is a common identifier, but it is not unique to an individual, as multiple patients may share the same birth date. While it is useful in conjunction with other identifiers, on its own, it does not provide a definitive identification.
Choice B reason:
The record number, often referred to as the medical record number, is a unique identifier assigned to each patient within a healthcare system. It is used to track patient information across different visits and services, making it the most reliable standalone identifier if the patient's name is not available.
Choice C reason:
An address can change over time and may not be unique, especially in large apartment buildings or for patients who are homeless or transient. It is also not specific enough to reliably identify a patient, as multiple individuals can reside at the same address.
Choice D reason:
The social security number is a unique identifier, but due to privacy concerns and the risk of identity theft, it is not the preferred method for patient identification in healthcare settings. Additionally, not all patients may have a social security number, such as non-residents or newborns.
Correct Answer is A
Explanation
Choice A reason:
The standard recommendation for fasting before a blood specimen collection is typically between 8 to 12 hours¹²³. This duration is considered sufficient to ensure that food intake does not affect the blood test results, such as glucose or lipid levels.
Choice B reason:
A fasting period of 1 to 2 hours is too short and would not provide an accurate representation of the body's baseline levels for most tests that require fasting. It is important for certain levels, like blood sugar and lipids, to stabilize, which takes longer than 1 to 2 hours.
Choice C reason:
Fasting for 4 to 6 hours may be appropriate for some tests, but it is not the standard fasting duration for most blood tests that require fasting. The shorter time might not allow for the clearance of lipids and glucose from the bloodstream that can influence the test outcomes.
Choice D reason:
A 24-hour fast is generally not required for routine fasting blood tests and could be potentially harmful, as it may lead to hypoglycemia, especially in diabetic patients or those with metabolic disorders. It is excessive for the purposes of standard blood tests.
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