When a serum separation tube (SST) is collected, which of the following actions should the phlebotomist take to ensure optimal test results?
Invert the tube 5 to 8 times and then set for 30 minutes prior to centrifugation.
Invert the tube 1 to 3 times and then set for 1 hour prior to centrifugation.
Invert the tube 2 to 4 times and then set for 15 minutes prior to centrifugation.
Invert the tube 8 to 10 times and then set for 2 hours prior to centrifugation.
The Correct Answer is A
Choice A reason:
Inverting the tube 5 to 8 times ensures that the clot activator mixes thoroughly with the blood, promoting proper clotting. Setting the tube aside for 30 minutes allows sufficient time for the blood to clot before centrifugation. This is the recommended procedure to ensure that the serum is properly separated from the clot, resulting in a quality specimen suitable for testing.
Choice B reason:
Inverting the tube only 1 to 3 times may not be enough to mix the clot activator with the blood adequately, which could lead to incomplete clotting and potentially compromised test results. Additionally, setting the tube for 1 hour before centrifugation is longer than necessary and does not offer any advantage over the recommended 30 minutes.
Choice C reason:
Inverting the tube 2 to 4 times might not fully mix the clot activator with the blood, and setting it for only 15 minutes does not provide enough time for proper clot formation. This could result in a suboptimal separation of serum and clot, affecting the integrity of the test results.
Choice D reason:
Inverting the tube 8 to 10 times could potentially cause hemolysis or disruption of the blood cells due to excessive agitation. Setting the tube for 2 hours prior to centrifugation is not recommended as it could lead to serum degradation or other changes that may affect the test results.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is B
Explanation
Choice A Reason:
Submitting laboratory results to the representative for workers' compensation is generally not a violation of patient privacy. This action is often necessary as part of the claims process and is typically done with the patient's consent or as mandated by law.
Choice B Reason:
Discussing laboratory results with a patient's partner without the patient's explicit consent is a violation of patient privacy. Confidentiality is a fundamental principle in healthcare, and disclosing health information to unauthorized individuals, including family members, breaches that confidentiality.
Choice C Reason:
Providing diagnosis codes to a patient's insurance company is a standard procedure and is not considered a violation of patient privacy. This information is necessary for the insurance company to process claims and provide coverage for medical services.
Choice D Reason:
Printing a patient's full name on the specimen label is standard practice and does not violate patient privacy. This is done to ensure that the specimen is correctly identified and matched to the patient, which is critical for accurate diagnosis and treatment.
Correct Answer is C
Explanation
Choice A Reason:
Povidone-iodine is an antiseptic used for skin disinfection before and after surgery. It has a broad spectrum of antimicrobial activity, but it is not the preferred choice for capillary blood collection due to the potential interference with certain laboratory tests. Povidone-iodine needs to be completely dried to avoid hemolysis of the sample, which can affect test results.
Choice B Reason:
Hand sanitizer is generally used for hand hygiene and not for the disinfection of the site for blood specimen collection. While it is effective in killing germs on the skin, it contains emollients and gelling agents that can contaminate the blood sample and interfere with laboratory tests.
Choice C Reason:
70% isopropyl alcohol is the most commonly used disinfectant for aseptic technique in capillary blood collection. It is effective against a broad range of microorganisms and evaporates quickly, leaving no residue that could contaminate the sample. It is important to allow the alcohol to dry completely before performing the puncture to prevent stinging and sample dilution.
Choice D Reason:
A 10% chlorine bleach solution is not typically used for skin disinfection in blood collection due to its harshness and potential to cause skin irritation and damage. It is more commonly used for disinfecting surfaces and equipment in healthcare settings.
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