Which of the following methods should a phlebotomist use to invert tubes to prevent clotting?
Shaking the tubes 4 to 8 times after collection.
Moving the tubes in a figure-eight motion 4 to 8 times after collection.
Rolling the tubes between the palms of the hands 4 to 8 times after collection.
Passing the tubes from hand to hand 4 to 8 times after collection.
The Correct Answer is C
Choice A Reason:
Shaking the tubes vigorously can cause hemolysis, which is the rupture of red blood cells, leading to inaccurate test results. It is not recommended as it does not provide gentle mixing and can damage the blood cells.
Choice B Reason:
Moving the tubes in a figure-eight motion is not a standard practice for mixing blood samples. This method may not provide the consistent and gentle inversion needed to mix the anticoagulant with the blood effectively.
Choice C Reason:
Rolling the tubes between the palms of the hands 4 to 8 times after collection is the recommended method. This technique allows for gentle and thorough mixing of the blood with the anticoagulant, preventing clotting without causing hemolysis. The number of inversions ensures that the anticoagulant coats the inner surface of the tube and mixes with the blood, which is essential for accurate laboratory results.
Choice D Reason:
Passing the tubes from hand to hand is not an effective method for mixing blood samples. This action may not provide the necessary inversion and can lead to inadequate mixing, resulting in clotted specimens.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
Choice A Reason:
When a patient is under "Contact Precautions," it is recommended that healthcare workers don a gown upon entering the patient's room. This is to prevent the spread of infection through contact with the patient or their environment. The gown should be removed before leaving the room to avoid contaminating other areas.
Choice B Reason:
While a face shield may be necessary for certain procedures, it is not specifically required for "Contact Precautions." The primary concern with contact precautions is preventing the transmission of infections through touch, not through droplets, which is what a face shield would protect against.
Choice C Reason:
Donning a mask is not a standard requirement for "Contact Precautions" unless there is a risk of droplet transmission. In this scenario, the precautions are specifically for contact, and therefore, a mask alone would not be sufficient.
Choice D Reason:
Gloves should be donned before any potential contact with the patient or their immediate environment, not just after cleaning and evaluating the venipuncture site. Gloves are a critical component of PPE when dealing with patients under "Contact Precautions."
Correct Answer is D
Explanation
Choice A Reason:
Inverting the tube 5 to 7 times may not be sufficient to mix the blood thoroughly with the EDTA. This could lead to partial clotting and potentially inaccurate test results. The EDTA anticoagulant works by binding calcium ions, which are necessary for blood clotting. Without adequate mixing, the EDTA may not be evenly distributed, leaving some areas of the blood sample able to clot.
Choice B Reason:
Inverting the tube only 2 to 4 times is inadequate for proper mixing. This minimal agitation would likely result in clot formation because the anticoagulant would not be sufficiently mixed with the blood. Clots can interfere with the accuracy of hematological tests by trapping cells and altering the specimen's composition.
Choice C Reason:
Inverting the tube 1 to 3 times is clearly insufficient and would almost certainly lead to clotting. The purpose of inverting the tube is to ensure that the EDTA coats all the blood cells, preventing coagulation. Such a low number of inversions would not allow for the anticoagulant to perform its function effectively.
Choice D Reason:
Inverting the tube 8 to 10 times is the recommended practice. This number of inversions ensures that the blood is fully mixed with the EDTA, preventing clot formation and preserving the integrity of the sample for accurate laboratory analysis. It is important to perform these inversions gently to avoid hemolysis, which can also affect test results. Hemolysis occurs when red blood cells are damaged and their contents leak out, which can happen if the blood is shaken too vigorously.
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